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Product Details
Materials Provided
IDComponentsSizeEP149-C01High-bind Plate1 plateEP149-C02Human IL-1355 μgEP149-C03Biotinylated Human IL-13RA210 μgEP149-C04Anti-IL-13RA2 Neutralizing Antibody20 μgEP149-C05Streptavidin-HRP10 μgEP149-C06Coating Buffer12 mLEP149-C0710xWashing Buffer50 mLEP149-C08Blocking Buffer50 mLEP149-C09Substrate Solution12 mLEP149-C10Stop Solution7 mLProduct Overview
IL-13RA2 [Biotinylated] : IL-13 Inhibitor Screening ELISA Kit is based on a competition-ELISA method, is used to screening for inhibitors of human IL-13RA2 binding to human IL-13. The kit is designed to provide a reliable solution for the screening of antibodies and small molecule inhibitors in the early stages, as well as QC release, to help researchers quickly screen and validate drugs, effectively improving research and development efficiency. It can also be used as a universal detection tool to identify the ability of human IL-13RA2 binding to human IL-13.
Reconstitution
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
INHIBITION OF IL-13RA2[Biotinylated]: IL-13 BINDING BY ANTI-IL-13RA2 NEUTRALIZING ANTIBODY
Serial dilutions of Anti-IL-13RA2 Neutralizing antibody (Catalog # EP149-C04) (1:1 serial dilution, from 10 μg/mL to 0.039μg/mL) was added into Biotinylated IL-13RA2:IL-13 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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