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Human TL1A-DR3 inhibition Kit (TR-FRET)

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FAB
  1. Cost effective - Sufficient quantity at a lower price, accounting for dilution and pipetting losses.
  2. Comprehensive validation - Validated with various antibody subtypes and antibody drugs.
  3. Simple and fast operation - No complicated washing steps, significantly reducing time.
  4. High batch consistency - Strict control over raw materials and finished product quality, ensuring a stable supply.
  5. Accurate and reliable results - High sensitivity with minimal matrix effects.
  6. High throughput capability - Supports 500 tests, ideal for high-throughput screening.
  7. Fast completion - Results in just 1 hour.
    Product Specifications
    Assay TypeInhibition-TR-FRET
    AnalyteAnti-TL1A Neutralizing Antibody
    Format100T/500T
    ReactivityHuman
    Regulatory StatusRUO
    SensitivityIC50=2.816nM
    Standard Curve Range0.1302 nM-66.6667 nM
    Assay Time1 hr
    Suitable Sample TypeFor screening assay of neutralizing antibodies binding to the human TL1A
    Sample volume10 μL
  • Product Overview
    The Human TL1A-DR3 inhibition Kit (TR-FRET) is based on a homogeneous (no wash) competition TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer) to screening for inhibitors of human TL1A binding to human DR3 within 0.5-1 hours. It can also be used as a universal detection tool to identify the ability of TL1A to bind to human DR3.
  • Storage
    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    Materials Provided
    IDComponentsSize
    FRT02-C01Human TL1A Protein Europium-chelate100 tests/500 tests
    FRT02-C02FA Labeled Human DR3 Protein100 tests/500 tests
    FRT02-C03Monoclonal Anti-TL1A Antibody, Human IgG120 μg/100 tests
    100 μg/500 tests
    FRT02-C04Sample Dilution Buffer10 mL/100tests & 500tests
    FRT02-C05Detection Buffer10 mL/100tests & 500tests
  • Assay Principles
    This Human TL1A-DR3 inhibition kit (TR-FRET) is based on TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer). Use a mixture of biotinylated human TL1A and Europium-chelate labeled streptavidin as the donor and FA labeled human DR3 protein as the acceptor.
    - In the absence of inhibitors for human TL1A binding to human DR3, the donor and acceptor are in close proximity due to the binding of human TL1A and FA-labeled human DR3. Upon excitation with a specific light source, the donor emits a 620 nm signal, which is absorbed by the acceptor, resulting in a 665 nm emission.
    - In the presence of inhibitors that block the binding of human TL1A to human DR3, the donor-acceptor interaction is disrupted, preventing FRET from occurring.
Bioactivity-TR-FRET Please refer to DS document for the assay protocol.
 TL1A-DR3 TR-FRET

Inhibition of Europium-chelate labeled human TL1A: FA labeled human DR3 binding by Human Anti-TL1A Neutralizing Antibody
Premix serial dilutions of Human Anti-TL1A Neutralizing Antibody (1:2 serial dilution, from 10 μg/mL to 0.01953125 μg/mL (66.6667-0.1302 nM)) and Human TL1A Protein Europium-chelate and incubate at room temperature (20℃-25℃) for 0.5 hours. Then add FA Labeled Human DR3 Protein and incubate at room temperature (20℃-25℃) for 0.5 hours. Detection was performed with IC50 of 2.816nM. The assay was performed according to the above-described Datasheet (QC tested).

  • Clinical and Translational Updates

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