Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09), CF (carrier free), premium grade

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218-MY23G0-500ug
$2,811.00
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218-MY23G0
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Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09), CF (carrier free), premium grade is a monoclonal antibody recombinantly expressed from HEK293 cells, which provides higher batch consistency and long term security of supply.

It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.

Isotype

Rabbit IgG, Kappa

Application

Flow Cytometry (Evaluation of CAR and SCFV containing 218linker on Human cells).

Specificity

This product is a specific antibody specifically reacts with CAR or scFv containing the 218 linker sequence.

Endotoxin

Less than 0.01 EU per μg by the LAL method / rFC method.

Protein A

<5 ppm of protein tested by ELISA.

Host Cell Protein

<0.5 ng/μg of protein tested by ELISA.

Host Cell DNA

<0.02 ng/μg of protein tested by qPCR.

Purity

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Sterility

Negative

Mycoplasma

Negative

Formulation

Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Shipping

This product is supplied and shipped with dry ice, please inquire the shipping cost.

Storage

For long term storage, the product should be stored at liquid state at -70°C.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • 2-8°C for 12 months under sterile conditions;
  • -70°C for 24 months.

Introduction de fond

The whitlow/218 linker, with the sequence GSTSGSGKPGSGEGSTKG, is a commonly used synthetic peptide linker. Whitlow/218 linker often leveraged to connect the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The scFv containing the 218 linker showed reduced aggregation and was found to be more stable to proteolysis in vitro.