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Détails du produit
Synonyms
EGFR, ERBB, ERBB1, HER1, PIG61, mENA
Source
Mouse EGF R Protein, Fc Tag (EGR-M5257) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 647 (Accession # Q01279-1).
Predicted N-terminus: Leu 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 95.7 kDa. The protein migrates as 110-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Mouse EGF R Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Mouse EGF R Protein, Fc Tag (Cat. No. EGR-M5257) is more than 90% and the molecular weight of this protein is around 215-245 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Mouse EGF R Protein, Fc Tag (Cat. No. EGR-M5257) at 5 μg/mL (100 μL/well) can bind Human EGF, Mouse IgG2a Fc Tag (Cat. No. EGF-H525b) with a linear range of 0.5-63 ng/mL (QC tested).
Protocol
Immobilized Human EGF, Mouse IgG2a Fc Tag (Cat. No. EGF-H525b) at 2 μg/mL (100 μL/well) can bind Mouse EGF R Protein, Fc Tag (Cat. No. EGR-M5257) with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
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Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Epidermal growth factor receptor erbB1
- Catégorie：
- Nombre de médicaments répertoriés：
39 Détails
- Nombre de médicaments cliniques：
206 Détails
- Étape de développement la plus élevée：
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