Endo H (500U/μl)

Endo H is a recombinant glycosidase cloned from Streptomyces plicatus and overexpressed in E. coli. It cleaves the chitobiose core of high-mannose oligosaccharides and a limited number of hybrid oligosaccharides from asparagine-linked glycoproteins.

Endo H cleaves within the chitobiose core of high mannose and some hybrid oligosaccharides from N-linked glycoproteins.

One unit is defined as the amount of enzyme required to remove >95% of the carbohydrate from 10µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10µl.

>95% as determined by SDS-PAGE.

>500 U/μL

Supplied as 0.2 μm filtered solution in 20 mM Tris, 50 mM NaCl, pH7.5.

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This product is supplied and shipped on dry ice.

• The product MUST be stored at -20°C or lower upon receipt.
• -20°C for 12 months under sterile conditions.