Human Erythropoietin R & CD131 Protein, Fc Tag&Fc Tag (MALS verified)

Synonyms

Erythropoietin R & CD131

Source

Human Erythropoietin R & CD131 Protein, Fc Tag&Fc Tag (ERR-H5255) is expressed from human 293 cells (HEK293). It contains AA Ala 25 - Pro 250 & Trp 17 - Trp 443 (Accession # P19235-1 & P32927-1).

Predicted N-terminus: Ala 25 & Trp 17

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

Human Erythropoietin R & CD131 Protein, Fc Tag&Fc Tag is produced by co-expression of Erythropoietin R and CD131, has a calculated MW of 51.3 kDa (Erythropoietin R) & 74.9 kDa (CD131). Subunit Erythropoietin R is fused with a human IgG1 Fc tag at the C-terminus and subunit CD131 is fused with a human IgG1 Fc tag at the C-terminus. The protein migrates as 55-60 kDa and 80-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

The protein is designed as a heterodimer.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process