Collectionner le produit
Détails du produit
Synonyms
FZD7, Frizzled-7, FzE3, Fz-7, hFz7
Source
Human Frizzled-7, His Tag (FZ7-H52H9) is expressed from human 293 cells (HEK293). It contains AA Gln 33 - Leu 185 (Accession # O75084-1).
Predicted N-terminus: Gln 33
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 18.6 kDa. The protein migrates as 25-32 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Les clients ont également consulté
Affichage des données
SDS-PAGE
Human Frizzled-7, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human Frizzled-7, His Tag (Cat. No. FZ7-H52H9) at 5 μg/mL (100 μL/well) can bind Wnt surrogate Human IgG1(D239E,L241M) | Human Lambda with a linear range of 1-16 ng/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human Glypican 3, His,Avitag, premium grade (Cat. No. GP3-H82E5) on SA Biosensor, can bind Human Frizzled-7, His Tag (Cat. No. FZ7-H52H9) with an affinity constant of 1.18 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Avis des utilisateurs Publier un commentaire
Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Frizzled-7
- Catégorie：
- Nombre de médicaments répertoriés：
0 Détails
- Nombre de médicaments cliniques：
1 Détails
- Étape de développement la plus élevée：
Phase 1 Clinical
Contact Us
Drug Candidate Licensing
