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HLA-DRA1*0101 & HLA-DRB1*1101
Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*11:01 Monomer Protein (HL1-H82W6) is expressed from human 293 cells (HEK293). It contains AA Ile 26 - Glu 216 (HLA-DRA1*01:01) & Gly 30 - Lys 227 (HLA-DRB1*11:01) (Accession # CAI2388006.1 (HLA-DRA1*01:01) & CAI2388099.1 (HLA-DRB1*11:01)).
Predicted N-terminus: Ile 26 & Gly 30
Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*11:01 Monomer Protein, produced by co-expression of HLA-DRA1*01:01 and HLA-DRB1*11:01, has a calculated MW of 29.2 kDa (HLA-DRA1*01:01) and 29.2 kDa (HLA-DRB1*11:01). Subunit HLA-DRA1*01:01 is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit HLA-DRB1*11:01 contains no tag. The protein migrates as 40-43 kDa and 35-38 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Passed as determined by the SABA assay / HABA assay / binding ELISA.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.