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IL-2 R beta & IL-2 R gamma, IL-2RB & IL-2RG
Biotinylated Human IL-2RB&IL-2RG Heterodimer Protein, Fc,Avitag&Fc,Avitag (ILG-H82F3) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 (IL-2RB) & Leu 23 - Asn 254 (IL-2RG) (Accession # P14784-1 (IL-2RB) & P31785-1 (IL-2RG)).
Predicted N-terminus: Ala 27 (IL-2RB) & Leu 23 (IL-2RG)
Biotinylated Human IL-2RB&IL-2RG Heterodimer Protein, Fc,Avitag&Fc,Avitag is produced by co-expression of IL-2RB and IL-2RG, has a calculated MW of 52.8 kDa (IL-2RB) and 55.3 kDa (IL-2RG). Subunit IL-2RB is fused with a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit IL-2RG is fused with a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™). The protein migrates as 60-66 kDa and 80-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
0.7-1.0 as determined by the SABA assay / HABA assay / binding ELISA.
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.