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Biotinylated Human IL-23 R Protein, His,Avitag™ (MALS & SPR verified)

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Numéro de produit/spécification
Prix
Qté
ILR-H82E8-25ug
$360.00
ILR-H82E8-200ug
$1565.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    IL-23 R

  • Source

    Biotinylated Human IL-23 R Protein, His,Avitag (ILR-H82E8) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Gly 355 (Accession # Q5VWK5-1).

    Predicted N-terminus: Gly 24

    Request for sequence
  • Molecular Characterization

    IL-23 R Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 41.6 kDa. The protein migrates as 60-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    IL-23 R SDS-PAGE

    Biotinylated Human IL-23 R Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IL-23 R SEC-MALS

    The purity of Biotinylated Human IL-23 R Protein, His,Avitag (Cat. No. ILR-H82E8) is more than 90% and the molecular weight of this protein is around 50-80 kDa verified by SEC-MALS.

    Report
  • Bioactivity-SPR

     IL-23 R SPR

    Biotinylated Human IL-23 R Protein, His,Avitag (Cat. No. ILR-H82E8) immobilized on SA Chip can bind Human IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-H52W5) with an affinity constant of 3.76 nM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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Introduction de fond

Interleukin-23 subunit alpha (IL-23 alpha) can associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

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