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Biotinylated Mouse IL-6 Protein, Avitag™,His Tag

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Numéro de produit/spécification
Prix
Qté
IL6-M82Q7-25ug
$360.00
IL6-M82Q7-200ug
$1565.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    IL6, Interleukin-6, BSF2, HSF, IFNB2

  • Source

    Biotinylated Mouse IL-6, Avitag,His Tag (IL6-M82Q7) is expressed from human 293 cells (HEK293). It contains AA Phe 25 - Thr 211 (Accession # P08505-1).

    Predicted N-terminus: Gly

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  • Molecular Characterization

    IL-6 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 25.3 kDa. The protein migrates as 28-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    IL-6 SDS-PAGE

    Biotinylated Mouse IL-6, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Bioactivity-ELISA

     IL-6 ELISA

    Immobilized Biotinylated Mouse IL-6, Avitag,His Tag (Cat. No. IL6-M82Q7) at 1 μg/mL (100 μL/well) on streptavidin precoated (STN-N5116) can bind Mouse IL-6 R alpha Protein, Fc Tag (Cat. No. ILR-M5255) with a linear range of 0.3-2 ng/mL (QC tested).

    Protocol

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Introduction de fond

Interleukin 6 (IL-6) is also known as HGF, BSF2,HSF, IFNB2 and IL-6, originally identified as a B cell differentiation factor, is a multifunctional cytokine that regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions.It is secreted by T cells, macrophages , monocytes, fibroblasts,endothelial cells,et.al. to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein. IL-6 is relevant to many disease processes such as diabetes,atherosclerosis, depression,Alzheimer's Disease,systemic,lupus erythematosus,prostate cancer and rheumatoid arthritis. Advanced/metastatic cancer patients have higher levels of IL-6 in their blood.Hence there is an interest in developing anti-IL-6 agents as therapy against many of these diseases.

Progrès de frontière

 
Progrès du développement médicamenteux
  • Nom anglais:

    Interleukin-6

  • Catégorie:

  • Nombre de médicaments répertoriés:

    5 Détails

  • Nombre de médicaments cliniques:

    22 Détails

  • Étape de développement la plus élevée:

    Approved

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