Détails du produit
Synonyms
IL-15, CD215
Source
Human IL15RA & IL15 Protein, Fc Tag (N120D) (IL5-H5256) is expressed from human 293 cells (HEK293). It contains AA Ile 31 - Arg 95(IL15RA) & Asn 49 - Ser 162(IL15) (Accession # Q13261-1 & P40933-1(N120D)).
Predicted N-terminus: Ile 31
Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 48.0 kDa. The protein migrates as 53-63 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE

Human IL15RA & IL15 Protein, Fc Tag (N120D) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA

Immobilized Human IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag (Cat. No. ILG-H5283) at 2 μg/mL (100 μL/well) can bind Human IL15RA & IL15 Protein, Fc Tag (N120D) (Cat. No. IL5-H5256) with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
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