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Human KIR2DL5 / CD158f Protein, Fc Tag

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Numéro de produit/spécification
Prix
Qté
KIA-H5255-100ug
$380.00
KIA-H5255-1mg (500ug X 2)
$2380.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    CD158f

  • Source

    Human KIR2DL5 / CD158f Protein, Fc Tag (KIA-H5255) is expressed from human 293 cells (HEK293). It contains AA His 22 - His 240 (Accession # Q8N109-1).

    Predicted N-terminus: His 22

    Request for sequence
  • Molecular Characterization

    KIR2DL5A Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 50 kDa. The protein migrates as 63-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    KIR2DL5A SDS-PAGE

    Human KIR2DL5 / CD158f Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Bioactivity-SPR

     KIR2DL5A SPR

    Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Complex Protein (Cat. No. HLM-H82E4) immobilized on CM5 Chip can bind Human KIR2DL5 / CD158f Protein, Fc Tag (Cat. No. KIA-H5255) with an affinity constant of 27.7 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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Introduction de fond

Killer cell immunoglobulin-like receptor 2DL5(KIR2DL5), which belongs to the immunoglobulin superfamily, is an inhibitory receptor of NK cells. KIR2DL5 is encoded by two paralogous genes displaying copy number variation and allelic polymorphism-KIR2DL5A and KIR2DL5B that vary by only 2 aa in domain and 1 aa in signal peptide. KIR2DL5 and KIR2DL4 form a subfamily, which have longer cytoplasmic tails than other KIR, and each has one D0 and one D2-type Ig-like domain.

Progrès de frontière

 
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