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Cynomolgus / Rhesus macaque Klotho beta Protein, His Tag

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Numéro de produit/spécification
Prix
Qté
KLB-R52H8-100ug
$360.00
KLB-R52H8-1mg (500ug X 2)
$2380.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonyms

    betaKlotho, beta-klotho, BKL, KLB, klotho beta like, klotho beta-like protein

  • Source

    Cynomolgus / Rhesus macaque Klotho beta Protein, His Tag (KLB-R52H8) is expressed from human 293 cells (HEK293). It contains AA Phe 53 - Leu 997 (Accession # G7MSR8-1). In the region Phe 53 - Leu 997, the amino acid sequences of Cynomolgus and Rhesus macaque Klotho beta are identical.

    Predicted N-terminus: Phe 53

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  • Molecular Characterization

    Klotho beta Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 110.3 kDa. The protein migrates as 115-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    Klotho beta SDS-PAGE

    Cynomolgus / Rhesus macaque Klotho beta Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Bioactivity-BLI

     Klotho beta BLI

    Loaded Human FGF R4, Fc Tag (Cat. No. FG4-H5253) on Protein A Biosensor, can bind Cynomolgus / Rhesus macaque Klotho beta Protein, His Tag (Cat. No. KLB-R52H8) with an affinity constant of 0.9 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

    Protocol
  •  Klotho beta BLI

    Loaded Human FGF19, Fc Tag (Cat. No. FG9-H5253) on Protein A Biosensor, can bind Cynomolgus / Rhesus macaque Klotho beta Protein, His Tag (Cat. No. KLB-R52H8) with an affinity constant of 78.5 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

    Protocol

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Introduction de fond

KLB (Klotho Beta) is a Protein Coding gene. Among its related pathways are RET signaling and HIV Life Cycle. GO annotations related to this gene include hydrolase activity, hydrolyzing O-glycosyl compounds and fibroblast growth factor binding. An important paralog of this gene is KL. Klotho Beta is a regulator in multiple metabolic processes, while its role in cancer remains unclear. We found the expression of βKlotho was down-regulated in human hepatocellular carcinoma tissues compared with that in paired adjacent non-tumourous liver tissues. Hepatoma cells also showed decreased expression of βKlotho compared with normal hepatocyte cells. Reintroduction of βKlotho into hepatoma cells inhibited their proliferation.

Progrès de frontière

 
Progrès du développement médicamenteux
  • Nom anglais:

    Klotho beta

  • Catégorie:

  • Nombre de médicaments répertoriés:

    0 Détails

  • Nombre de médicaments cliniques:

    3 Détails

  • Étape de développement la plus élevée:

    Phase 2 Clinical

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