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Human MMP-9 Protein, His Tag (active enzyme) (MALS verified)

COADS MS
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Cat. No. / Size
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MM9-H5229-50ug
$360.00
MM9-H5229-1mg
$2245.00
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Product Details

  • Synonym

    MMP9, CLG4B, GELB, MANDP2, Gelatinase B

  • Source

    Human MMP-9, His Tag (MM9-H5229) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Pro 469 (Accession # AAH06093.1). It needs to be activated by agents such as APMA in vitro to have hydrolytic activity.

    Predicted N-terminus: Ala 20

    Request for sequence

  • Molecular Characterization

    MMP-9 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 50.8 kDa. The protein migrates as 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Supplied as 0.2 μm filtered solution in 25 mM Tris, 150 mM NaCl, 20% glycerol, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    MMP-9 SDS-PAGE

    Human MMP-9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    MMP-9 SEC-MALS

    The purity of Human MMP-9, His Tag (Cat. No. MM9-H5229) is more than 90% and the molecular weight of this protein is around 50-70 kDa verified by SEC-MALS.

    Report

  • Bioactivity

    Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2. The specific activity is >3,000 pmol/min/μg (QC tested).

    Protocol

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Background

Matrix metallopeptidase 9 (MMP-9) is also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB), CLG4B, is secreted from neutrophils, macrophages, and a number of transformed cells, and is the most complex family member in terms of domain structure and regulation of its activity. Structurally, MMP9 maybe be divided into five distinct domains: a prodomain which is cleaved upon activation, a gelatinbinding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a prolinerich linker region, and a carboxyl terminal hemopexinlike domain. This enzyme degrades various substrates including gelatin, collagen types IV and V, and elastin. MMP9 is involved in a variety of autoimmune diseases such as systemic lupus erythematosus, rheumatoid arthritis, and multiple sclerosis, and be regarded as a potential therapeutic target.

Recent Advances

 
Drug Development Progress
  • English Name：

    Matrix metalloproteinase 9

  • Category：

  • Approved Drugs：

    0 Details

  • Drugs in Clinical Trials：

    3 Details

  • Highest Development Stage：

    Phase 3 Clinical

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