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Détails du produit
Synonyms
NKG2A, CD159a, KLRC1, NK cell receptor A
Source
Human NKG2A, His Tag (NKA-H5244) is expressed from human 293 cells (HEK293). It contains AA Pro 94 - Leu 233 (Accession # P26715-1).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 17.9 kDa. The protein migrates as 30-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Affichage des données
SDS-PAGE
Human NKG2A, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Avis des utilisateurs Publier un commentaire
A***n2024-09-17
J'aimeI used this recombinant protein to test function of a gRV transduction on iPS-derived cells. The product went into solution quickly during reconstitution and provided expected results from positive controls and testing samples. Signal was clear on a flow cytometry based readout using Streptavidin-Brilliant Violet 711. I used this at 5 ug at a concentration of 100 ug/mL on 50,000 total cells. No support was needed, as the provided data sheet and CoA are thorough and easy to follow. Very satisfied with my experience.
Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
NK cell lectin-like receptor subfamily C member 1
- Catégorie：
- Nombre de médicaments répertoriés：
0 Détails
- Nombre de médicaments cliniques：
6 Détails
- Étape de développement la plus élevée：
Phase 3 Clinical
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