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Détails du produit
Synonyms
Spike, S protein, Spike glycoprotein, S glycoprotein
Source
SARS-CoV-2 Spike Trimer Protein, His Tag (EG.5/Omicron) (SPN-C524u) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (T19I, LPP24-26del, A27S, V83A, G142D, Y144del, H146Q, Q183E, V213E, G252V, G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, F456L, N460K, S477N, T478K, E484A, F486P, F490S, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K, R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)). The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: EG.5). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
Predicted N-terminus: Val 16
Request for sequenceMolecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 137.8 kDa. The protein migrates as 180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
SARS-CoV-2 Spike Trimer Protein, His Tag (EG.5/Omicron) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of SARS-CoV-2 Spike Trimer Protein, His Tag (EG.5/Omicron) (Cat. No. SPN-C524u) is more than 85% and the molecular weight of this protein is around 530-580 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike Trimer Protein, His Tag (EG.5/Omicron) (Cat. No. SPN-C524u) with a linear range of 0.977-31.25 ng/mL (QC tested).
Protocol
Immobilized SARS-CoV-2 Spike Trimer Protein, His Tag (EG.5/Omicron) (Cat. No. SPN-C524u) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.122-3.906 ng/mL (Routinely tested).
Protocol
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Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Coronavirus spike glycoprotein
- Catégorie：
- Nombre de médicaments répertoriés：
15 Détails
- Nombre de médicaments cliniques：
88 Détails
- Étape de développement la plus élevée：
Approved
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