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Détails du produit
Synonyms
RANKL, CD254, TRANCE, OPGL, ODF
Source
Human TNFSF11, Fc Tag (RAL-H5265) is expressed from human 293 cells (HEK293). It contains AA Gly 64 - Asp 245 (Accession # AAC51762.1).
Predicted N-terminus: Pro
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 46.9 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Human TNFSF11, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-ELISA
Immobilized Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251) at 0.5 μg/mL (100 μL/well) can bind Human TNFSF11, Fc Tag (Cat. No. RAL-H5265) with a linear range of 0.1-3 ng/mL (QC tested).
Protocol
Immobilized Human OPG, His Tag (Cat. No. TNB-H5220) at 10 μg/mL (100 μL/well) can bind Human TNFSF11, Fc Tag (Cat. No. RAL-H5265) with a linear range of 0.3-2 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Captured Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251) on CM5 chip via Anti-Mouse antibodies surface can bind Human TNFSF11, Fc Tag (Cat. No. RAL-H5265) with an affinity constant of 0.574 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251) on AMC Biosensor, can bind Human TNFSF11, Fc Tag (Cat. No. RAL-H5265) with an affinity constant of 0.205 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
Tumor necrosis factor ligand superfamily member 11
- Catégorie：
- Nombre de médicaments répertoriés：
18 Détails
- Nombre de médicaments cliniques：
18 Détails
- Étape de développement la plus élevée：
Approved
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