Human ALK-7 (Activin E receptor) (Luc) HEK293 Reporter Cell

1. Genetically modified cell lines best reflect MOA (Mechanism of Action)
2. Higher activity and larger assay window for robust and reproducible cell-based bioassay
3. Comprehensive application data to support assay development and validation
4. Full tracible record, stringent quality control and validated cell passage stability
5. Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
6. Global commercial license assistance whenever regulatory filing is required

Description

The Human ALK-7 (Activin E receptor) (Luc) HEK293 Reporter Cell was engineered to not only express Smad signaling response element, but also express the receptor full length human ALK-7 (Uniprot: Q8NER5-1). When stimulated with human Activin E protein, receptor-mediated signaling can drive Smad-mediated luminescence. Inhibition of biological effect of human Activin E protein by corresponding inhibitors results in a decrease in luminescence.

Application

• Screen for neutralizing antibodies blocking the ligand-receptor interaction

• Screen for human ALK-7 small molecule inhibitor

Growth Properties

Adherent

Selection Marker

Puromycin (2 μg/mL) + Hygromycin B (20 μg/mL)

Complete Growth Medium

DMEM + 10% FBS

Freeze Medium

Serum-free cell cryopreservation medium

Quantity

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Storage

Frozen in liquid nitrogen.

Mycoplasma Testing

Negative

Sterility Testing

Negative

Instructions for Use

See data sheet for detailed culturing and assay protocol.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process

LIMITED USE AND LICENSE STATEMENT

HEK293 Cell Line Products

By using any HEK293 cell line product purchased from ACRO Biosystems or its affiliates (collectively, "ACROBiosystems"), including the original 293 cells and any modified derivatives thereof (collectively, the "Product"), you (the "Customer") agree to be bound by the following terms of limited use. If you are not willing to accept these terms and the Product remains unused, please contact our customer service at customerservice@acrobiosystems.com to arrange for return of the unused Product for a refund.

• 1. Scope of Use

  For the use of the Product worldwide (excluding Mainland China, Hong Kong, Macao and Taiwan regions), Customer may use the Product for (i) internal research; (ii) drug discovery and assay development; (iii) internal quality assurance testing for biologic drugs and vaccines; and (iv) lot releasing assay for biologic drugs and vaccines, whether conducted by a commercial or non-commercial entity. Unless a separate sublicense has been obtained, it is NOT permitted to use the Product in (i) product manufacturing; (ii) provision of service, information, or data to an unaffiliated third party for a fee; (iii) resale of the Product or its derivatives, whether or not such Product or derivatives are resold for use in research or (iv) other commercial use not expressly permitted above.

  
  

  For the use of the Product in Greater China region (including Mainland China, Hong Kong, Macao and Taiwan regions), Customer may use the Product for internal research use only. Unless a separate sublicense has been obtained, it is NOT permitted to use the Product for any Commercial Use. For the purpose of this paragraph, "Commercial Use" means any and all uses of this product and derivatives by a party for profit or other consideration and may include but is not limited to use in: (i) product manufacture; (ii) provision of service, information or data to a third party for a fee; (iii) resale of the product or its derivatives, whether or not such Product or derivatives are resold for use in research; (iv) quality assurance testing for biologic drugs and vaccines; (v) quality control assays; (vi) lot releasing assay of biologic drugs and vaccines.

  
  

  If you require a sublicense for any use beyond the limited use permitted herein, including but not limited to commercial use, CRO/service provider activities for a fee, or any product manufacturing activities, please contact the local sales team of ACROBiosystems for further details.

  
  

  The Product is not intended for any animal or human therapeutic purposes, nor for any direct human in vivo use. The Product shall not be used in or administered to humans.

• 2. Transfer, Distribution and Sublicensing Restrictions

  Unless expressly permitted under a separate written agreement with/via ACROBiosystems, you have NO right to (i) share, transfer, distribute, sell, resell, sublicense, or otherwise make the Product or its derivatives available for use to any third party, including but not limited to other researchers, laboratories, departments, research institutions, hospitals, universities, contract research organizations (CROs), contract development and manufacturing organizations (CDMOs), or any other commercial entities; or (ii) use the Product or its derivatives to perform fee-for-service activities or to provide services, information, or data to any third party for monetary or other consideration.

• 3. Modification and Derivatives

  Customers have No right to modify, engineer, genetically alter, or create derivatives of the Product. Any such modification or derivative requires a separate license and additional fees. Please contact our local sales team for further details.

• 4. No Warranty

  ACROBiosystems makes no warranties or representations of any kind, either expressed or implied, with respect to the merchantability, fitness or suitability of the Product for any particular purpose.

• 5. Limitation of Liability

  To the fullest extent permitted by law, in no event shall ACROBiosystems or its licensors be liable for any use by Customer of the Product, any derivative thereof, or any material treated therewith for any losses, costs, claims, damages, or liability of whatsoever kind or nature which may arise from or in connection with the use of the Product.

• 6. Compliance with Laws

  Customer agrees to use the Product in compliance with all applicable laws, regulations, and governmental guidelines applicable to the Product, any derivative thereof, and any materials treated therewith.

ACKNOWLEDGMENT

By using this Product, Customer acknowledges that he/she has read, understood, and agreed to be bound by the terms and conditions of this Limited Use and License Statement. If Customer does not agree to comply with these terms, Customer shall not open or use the Product and shall contact ACROBiosystems to arrange for return of the unused Product.