Détails du produit
- Genetically modified cell lines best reflect MOA (Mechanism of Action)
- Higher activity and larger assay window for robust and reproducible cell-based bioassay
- Comprehensive application data to support assay development and validation
- Full tracible record, stringent quality control and validated cell passage stability
- Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
- Global commercial license assistance whenever regulatory filing is required
Description
The Raji/Human CD20 Knockout Stable Cell Line was generated from Raji cells through targeted knockout of human CD20 (Gene ID: 931). The expression level of human CD20 was confirmed by flow cytometry. Mutated sequences of human CD20 produced by non-homologous end joining (NHEJ) were confirmed through genomic sequencing.
Application
• Useful for cell-based CD20 target-specific analysis
Growth Properties
suspension
Selection Marker
NA
Complete Growth Medium
RPMI Medium 1640 + 10% FBS
Freeze Medium
Serum-free cell cryopreservation medium
Quantity
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
Storage
Frozen in liquid nitrogen.
Mycoplasma Testing
Negative
Sterility Testing
Negative
Instructions for Use
See data sheet for detailed culturing and assay protocol.
ACRO Quality Management System
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Affichage des données
Receptor Assay

Expression analysis of human CD20 on Raji/Human CD20 Knockout Stable Cell Line by FACS.
Cell surface staining was performed on Raji/Human CD20 Knockout Stable Cell Line using PE-labeled anti-human CD20 antibody. The Raji cells were stained with PE-labeled anti-human CD20 antibody as the positive control cell. The Raji cells were stained with PE-labeled isotype control antibody as the negative control cell.Protocol
Expression analysis of human CD19 on Raji/Human CD20 Knockout Stable Cell Line by FACS.
Cell surface staining was performed on Raji/Human CD20 Knockout Stable Cell Line using PE-labeled anti-human CD19 antibody. The Raji/Human CD20 Knockout Stable Cell Line was stained with PE-labeled isotype control antibody as the negative control cell.Protocol
Sequencing Analysis

Genomic Sequencing of human CD20 in the Raji/Human CD20 Knockout Stable Cell Line.
Sanger sequencing was used for mutation analysis of human CD20. The sequencing results demonstrated that frameshift mutations were generated in the human CD20 gene in the Raji/Human CD20 Knockout Stable Cell Line.
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Déclaration importante
Limited Use&License Disclosure
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE FOLLOWING TERMS OF LIMITED USE OF THIS CELL LINE PRODUCT.
- If the researcher is not willing to accept the terms of limited use of this cell line product, and the product is unused, ACRO will accept return of the unused product.
- Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for profit or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research.
- This cell line is neither intended for any animal or human therapeutic purposes nor for any direct human in vivo use. You have no right to share, modify, transfer, distribute, sell, sublicense, or otherwise make the cell line available for use to other researchers, laboratories, research institutions, hospitals, universities, or service organizations.
- ACROBIOSYSTEMS MAKES NO WARRANTIES OR REPRESENTATIONS OF ANY KIND, EITHER EXPRESSED OR IMPLIED, WITH RESPECT TO THE SUITABILITY OF THE CELL LINE FOR ANY PARTICULAR USE.
- ACROBIOSYSTEMS ACCEPTS NO LIABILITY IN CONNECTION WITH THE HANDLING OR USE OF THE CELL LINE.
- Modifications of the cell line, transfer to a third party, or commercial use of the cell line may require a separate license and additional fees. Please contact order@acrobiosystems.com for further details.
Progrès de frontière
- Nom anglais:
B-lymphocyte antigen CD20
- Catégorie:
- Nombre de médicaments répertoriés:
35 Détails
- Nombre de médicaments cliniques:
132 Détails
- Étape de développement la plus élevée:
Approved




















