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Détails du produit
Synonym
FLJ18683, T3E, TCRE, CD3E, CD3-epsilon
Source
Alexa Fluor 488-Labeled Human CD3 epsilon Protein, His Tag (CDE-HA2H3) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (Accession # P07766-1). It is the Alexa Fluor™ 488 labeled form of Human CD3 epsilon, His Tag (Cat. No. CDE-H5223).
Predicted N-terminus: Asp 23
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.9 kDa. The protein migrates as 20-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Conjugate
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with AF488 using standard chemical labeling method. The residual AF488 is removed by molecular sieve treatment during purification process.
Protein Ratio
The AF488 to protein molar ratio is 1-2.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Affichage des données
SDS-PAGE
Alexa Fluor 488-Labeled Human CD3 epsilon Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Alexa Fluor 488-Labeled Human CD3 epsilon Protein, His Tag (Cat. No. CDE-HA2H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) (Cat. No. CDE-M531) with a linear range of 0.2-8 ng/mL (QC tested).
Protocol
Bioactivity-FACS
1e5 of Mouse Anti-CD3 antibody coupled beads (5.5μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD3 epsilon Protein, His Tag (Cat. No. CDE-HA2H3) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
Protocol
1e5 of Mouse Anti-CD3 antibody coupled beads (5.5μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD3 epsilon Protein, His Tag (Cat. No. CDE-HA2H3) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
Protocol
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Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
T-cell surface glycoprotein CD3 epsilon chain
- Catégorie：
- Nombre de médicaments répertoriés：
4 Détails
- Nombre de médicaments cliniques：
7 Détails
- Étape de développement la plus élevée：
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