Alexa Fluor™ 488-Labeled Mouse CD3 epsilon Protein, Fc Tag

Synonym

FLJ18683, T3E, TCRE, CD3E, CD3-epsilon

Source

Alexa Fluor 488-Labeled Mouse CD3 epsilon Protein, Fc Tag (CDE-MA256) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 108 (Accession # P22646). It is the Alexa Fluor™ 488 labeled form of Mouse CD3E, Fc Tag (Cat. No. CDE-M5256).

Predicted N-terminus: Asp 23

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 36 kDa. The protein migrates as 40-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Conjugate

AF488

Excitation Wavelength: 488 nm

Emission Wavelength: 517 nm

Labeling

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with AF488 using standard chemical labeling method. The residual AF488 is removed by molecular sieve treatment during purification process.

Protein Ratio

The AF488 to protein molar ratio is 1.5-2.5.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process