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Détails du produit
Synonym
CD4, CD4mut, LEU3
Source
Alexa Fluor 488-Labeled Human CD4 Protein, His Tag (CD4-HA225) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Trp 390 (Accession # AAH25782). It is the Alexa Fluor™ 488 labeled form of Human CD4, His Tag (Cat. No. LE3-H5228).
Predicted N-terminus: Lys 26
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 41.5 kDa.
Conjugate
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with AF488 using standard chemical labeling method. The residual AF488 is removed by molecular sieve treatment during purification process.
Protein Ratio
The AF488 to protein molar ratio is 2-4.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
Bioactivity-ELISA
Immobilized Alexa Fluor 488-Labeled Human CD4 Protein, His Tag (Cat. No. CD4-HA225) at 1 μg/mL (100 μL/well) can bind Anti-CD4 Antibody (Ibalizumab-IgG4) with a linear range of 0.1-8 ng/mL (Routinely tested). Labeling with fluorescent dyes did not affect their activity.
Protocol
Bioactivity-FACS
1e5 of Mouse Anti-CD4 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD4 Protein, His Tag (Cat. No. CD4-HA225) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
Protocol
1e5 of Mouse Anti-CD4 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD4 Protein, His Tag (Cat. No. CD4-HA225) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
Protocol
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Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
T-cell surface antigen CD4
- Catégorie：
- Nombre de médicaments répertoriés：
1 Détails
- Nombre de médicaments cliniques：
25 Détails
- Étape de développement la plus élevée：
Approved
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