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Alexa Fluor™ 488-Labeled Human CD4 Protein, His TagStar Staining

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Numéro de produit/spécification
Prix
Qté
CD4-HA2H7-25ug
$505.00
CD4-HA2H7-200ug
$3295.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonym

    CD4, CD4mut, LEU3

  • Source

    Alexa Fluor 488-Labeled Human CD4 Protein, His Tag (CD4-HA2H7) is produced via conjugation of AF488 to Human CD4 Protein, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human CD4 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Pro 396 (Accession # AAH25782).

    Predicted N-terminus: Lys 26

  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

  • Conjugate

    AF488

    Excitation Wavelength: 488 nm

    Emission Wavelength: 517 nm

  • Protein Ratio

    The AF488 to protein molar ratio is 0.9-1.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Affichage des données

  • Bioactivity-ELISA

     CD4 ELISA

    Immobilized Anti-CD4 Antibody (Ibalizumab-IgG4) at 1 μg/mL (100 μL/well) can bind Alexa Fluor 488-Labeled Human CD4 Protein, His Tag (Cat. No. CD4-HA2H7) with a linear range of 0.2-16 ng/mL (Routinely tested). Labeling with fluorescent dyes did not affect their activity.

    Protocol
  • Bioactivity-FACS

     CD4 FACS

    1e5 of Mouse Anti-CD4 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD4 Protein, His Tag (Cat. No. CD4-HA2H7) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).

    Protocol
  •  CD4 FACS

    1e5 of Mouse Anti-CD4 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD4 Protein, His Tag (Cat. No. CD4-HA2H7) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).

    Protocol

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Introduction de fond

CD4 is a glycoprotein that serves as an essential co-receptor on the surface of T lymphocytes (T cells), particularly helper T cells. It plays a critical role in the immune system by recognizing and binding to major histocompatibility complex (MHC) class II molecules on antigen-presenting cells (APCs), thereby facilitating T cell activation and immune response.

Progrès de frontière

 
Progrès du développement médicamenteux
  • Nom anglais:

    T-cell surface antigen CD4

  • Catégorie:

  • Nombre de médicaments répertoriés:

    1 Détails

  • Nombre de médicaments cliniques:

    25 Détails

  • Étape de développement la plus élevée:

    Approved

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