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Détails du produit
Synonym
CD4, CD4mut, LEU3
Source
FITC-Labeled Human CD4, Fc Tag (CD4-HF255) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Pro 396 (Accession # P01730). It is the FITC labeled form of Human CD4 Protein, Fc Tag (Cat. No. CD4-H5259).
Predicted N-terminus: Lys 26
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 67.7 kDa. The protein migrates as 67-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Conjugate
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
Protein Ratio
The FITC to protein molar ratio is 3-5.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Affichage des données
SDS-PAGE
FITC-Labeled Human CD4, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Immobilized Ibalizumab (Human IgG4) at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human CD4, Fc Tag (Cat. No. CD4-HF255) with a linear range of 20-313 ng/mL (QC tested).
Protocol
Immobilized HIV-1 [HIV-1/Clade C (16055)] GP120, His Tag (Cat. No. GP5-V15224) at 10 μg/mL (100 μL/well) can bind FITC-Labeled Human CD4, Fc Tag (Cat. No. CD4-HF255) with a linear range of 0.039-0.313 μg/mL (Routinely tested).
Protocol
Bioactivity-FACS
2e5 of CD4-CAR-293 cells transfected with anti-CD4-scFv were stained with 100 μL of 1 μg/mL of FITC-Labeled Human CD4 Protein, Fc Tag (Cat. No. CD4-HF255) and negative control protein respectively. FITC signal was used to evaluate the binding activity (QC tested).
Protocol
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Introduction de fond
Progrès de frontière
Progrès du développement médicamenteux- Nom anglais：
T-cell surface antigen CD4
- Catégorie：
- Nombre de médicaments répertoriés：
1 Détails
- Nombre de médicaments cliniques：
25 Détails
- Étape de développement la plus élevée：
Approved
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