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Product Details
Synonym
CD7, GP40, TP41, LEU-9, Tp40
Source
Alexa Fluor 647-Labeled Human CD7 Protein, His Tag (CD7-HA2H4) is produced via conjugation of AF647 to Human CD7 Protein, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human CD7 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Pro 180 (Accession # P09564-1).
Predicted N-terminus: Ala 26
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
Conjugate
AF647
Excitation Wavelength: 640 nm
Emission Wavelength: 672 nm
Protein Ratio
The AF647 to protein molar ratio is 0.7-0.8.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
Bioactivity-ELISA
Immobilized Alexa Fluor 647-Labeled Human CD7 Protein, His Tag (Cat. No. CD7-HA2H4) at 1 μg/mL (100 μL/well) can bind Anti-CD7 antibody, Mouse IgG1 with a linear range of 0.05-3 ng/mL (Routinely tested). Labeling with fluorescent dyes did not affect their activity.
Protocol
Bioactivity-FACS
1e5 of Mouse Anti-CD7 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 647-Labeled Human CD7 Protein, His Tag (Cat. No. CD7-HA2H4) and negative control protein respectively, AF647 signal was used to evaluate the binding activity (QC tested).
Protocol
1e5 of Mouse Anti-CD7 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 647-Labeled Human CD7 Protein, His Tag (Cat. No. CD7-HA2H4) and negative control protein respectively, AF647 signal was used to evaluate the binding activity (QC tested).
Protocol
Bioactivity-Stability
Binding activity of two different lots of Alexa Fluor 647-Labeled Human CD7 Protein, His Tag against Mouse Anti-CD7 antibody coupled beads (5.5 μm) was evaluated by flow cytometry. The result shows very high batch-to-batch consistency.
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Background
Recent Advances
Drug Development Progress- English Name：
T-cell antigen CD7
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
32 Details
- Highest Development Stage：
Phase 2 Clinical
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