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Alexa Fluor™ 647-Labeled Human CD8 alpha Protein, His TagStar Staining

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Numéro de produit/spécification
Prix
Qté
CDA-HA2H5-25ug
$450.00
CDA-HA2H5-200ug
$2803.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonym

    CD8A, CD8, Leu2, MAL, p32

  • Source

    Alexa Fluor 647-Labeled Human CD8 alpha Protein, His Tag (CDA-HA2H5) is produced via conjugation of AF647 to Human CD8 alpha Protein, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human CD8 alpha Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Asp 182 (Accession # P01732-1).

    Predicted N-terminus: Ser 22

  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

  • Conjugate

    AF647

    Excitation Wavelength: 640 nm

    Emission Wavelength: 672 nm

  • Protein Ratio

    The AF647 to protein molar ratio is 0.8-1.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Affichage des données

  • Bioactivity-FACS

     CD8 alpha FACS

    1e5 of Mouse Anti-CD8 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 647-Labeled Human CD8 alpha Protein, His Tag (Cat. No. CDA-HA2H5) and negative control protein respectively, AF647 signal was used to evaluate the binding activity (QC tested).

    Protocol
  •  CD8 alpha FACS

    1e5 of Mouse Anti-CD8 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 647-Labeled Human CD8 alpha Protein, His Tag (Cat. No. CDA-HA2H5) and negative control protein respectively, AF647 signal was used to evaluate the binding activity (QC tested).

    Protocol

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Introduction de fond

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.

Progrès de frontière

 
Progrès du développement médicamenteux
  • Nom anglais:

    Cluster of differentiation 8

  • Catégorie:

  • Nombre de médicaments répertoriés:

    0 Détails

  • Nombre de médicaments cliniques:

    18 Détails

  • Étape de développement la plus élevée:

    Phase 3 Clinical

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