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Détails du produit
- Genetically modified cell lines best reflect MOA (Mechanism of Action)
- Higher activity and larger assay window for robust and reproducible cell-based bioassay
- Comprehensive application data to support assay development and validation
- Full tracible record, stringent quality control and validated cell passage stability
- Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
- Global commercial license assistance whenever regulatory filing is required
Description
The Human DR3 (TL1A receptor) (Luc) Jurkat Reporter Cell was engineered to not only express the NF-κB signaling response element, but also express the receptor human DR3 (TL1A receptor) (Uniprot: Q93038-1). When stimulated with human TL1A protein, the TL1A/DR3 interaction drives NF-κB-mediated luminescence. Inhibition of TL1A binding to DR3 by either anti-TL1A or anti-DR3 antibodies results in a decrease in luminescence.
Application
• Screen for neutralizing antibodies blocking the stimulation of human TL1A protein.
Growth Properties
Suspension
Selection Marker
Hygromycin B (20 μg/mL)
Complete Growth Medium
RPMI-1640 + 10% FBS
Freeze Medium
Serum-free cell cryopreservation medium
Quantity
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
Storage
Frozen in liquid nitrogen.
Mycoplasma Testing
Negative
Sterility Testing
Negative
Instructions for Use
See data sheet for detailed culturing and assay protocol.
ACRO Quality Management System
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Affichage des données
Application
Inhibition of human TL1A protein-induced reporter activity.
This reporter cell was incubated with serial dilutions of antibodies in the presence of human TL1A protein (Cat. No. TLA-H5243) with a final concentration of 0.02 μg/mL. The EC50 of anti-human TL1A neutralizing antibody is approximately 0.018 μg/mL.Protocol
Signaling Bioassay
Response to human TL1A protein (RLU).
The Human DR3 (TL1A receptor) (Luc) Jurkat Reporter Cell was incubated with serial dilutions of human TL1A protein (Cat. No. TLA-H5243). The parental reporter cell NF-kB (Luc) Jurkat Reporter Cell (Cat. No. SCJUR-STF113) lacking human DR3 expression was used as the control. The EC50 of human TL1A protein determined on Human DR3 (TL1A receptor) (Luc) Jurkat Reporter Cell was approximately 0.014 μg/mL. Compared to the Human DR3 (TL1A receptor) (Luc) Jurkat Reporter Cell, the parental reporter cell NF-kB (Luc) Jurkat Reporter Cell lacking human DR3 expression exhibited no activation response to human TL1A protein stimulation.Protocol
Response to human TL1A protein (FOLD).
The Human DR3 (TL1A receptor) (Luc) Jurkat Reporter Cell was incubated with serial dilutions of human TL1A protein (Cat. No. TLA-H5243). The parental reporter cell NF-kB (Luc) Jurkat Reporter Cell (Cat. No. SCJUR-STF113) lacking human DR3 expression was used as the control. The max induction fold of human TL1A protein determined on Human DR3 (TL1A receptor) (Luc) Jurkat Reporter Cell was approximately 54.12. Compared to the Human DR3 (TL1A receptor) (Luc) Jurkat Reporter Cell, the parental reporter cell NF-kB (Luc) Jurkat Reporter Cell lacking human DR3 expression exhibited no activation response to human TL1A protein stimulation.Protocol
Passage Stability
Passage stability analysis by Signaling Bioassay.
The continuously growing Human DR3 (TL1A receptor) (Luc) Jurkat Reporter Cell was stimulated with serial dilutions of human TL1A protein (Cat. No. TLA-H5243). Human TL1A protein stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 10-23.Protocol
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Limited Use&License Disclosure
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE FOLLOWING TERMS OF LIMITED USE OF THIS CELL LINE PRODUCT.
- If the researcher is not willing to accept the terms of limited use of this cell line product, and the product is unused, ACRO will accept return of the unused product.
- Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for profit or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research.
- This cell line is neither intended for any animal or human therapeutic purposes nor for any direct human in vivo use. You have no right to share, modify, transfer, distribute, sell, sublicense, or otherwise make the cell line available for use to other researchers, laboratories, research institutions, hospitals, universities, or service organizations.
- ACROBIOSYSTEMS MAKES NO WARRANTIES OR REPRESENTATIONS OF ANY KIND, EITHER EXPRESSED OR IMPLIED, WITH RESPECT TO THE SUITABILITY OF THE CELL LINE FOR ANY PARTICULAR USE.
- ACROBIOSYSTEMS ACCEPTS NO LIABILITY IN CONNECTION WITH THE HANDLING OR USE OF THE CELL LINE.
- Modifications of the cell line, transfer to a third party, or commercial use of the cell line may require a separate license and additional fees. Please contact order@acrobiosystems.com for further details.
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