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Détails du produit
Synonym
KLK1, Kallikrein-1, KLKR, Klk6
Source
Human Kallikrein 1 (19-261) Protein, His Tag (KL1-H52H4) is expressed from human 293 cells (HEK293). It contains AA Pro 19 - Ser 261 (Accession # P06870-1).
Predicted N-terminus: Pro 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 28.3 kDa. The protein migrates as 39-42 kDa and 43-46 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
Formulation
Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Human Kallikrein 1 (19-261) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Human Kallikrein 1 (19-261) Protein, His Tag (Cat. No. KL1-H52H4) was greater than 95% as determined by SEC-HPLC.
Bioactivity
Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is >8000 pmol/min/µg (QC tested).
Protocol
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