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Détails du produit
Synonym
KLK2A2, hGK-1, hK2
Source
Human Kallikrein 2 Protein, His Tag (KA2-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ile 25 - Pro 261 (Accession # P20151-1).
Predicted N-terminus: Ile 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 28.0 kDa. The protein migrates as 28-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>85% as determined by SDS-PAGE.
Formulation
Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
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Affichage des données
SDS-PAGE
Human Kallikrein 2 Protein, His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 85% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human Kallikrein 2 Protein, His Tag (Cat. No. KA2-H52H3) at 1 μg/mL (100 μL/well) can bind Human KLK2 Antibody, IgG with a linear range of 0.06-2 ng/mL (Routinely tested).
Protocol
Immobilized Human Kallikrein 2 Protein, His Tag (Cat. No. KA2-H52H3) at 1 μg/mL (100 μL/well) can bind Anti-KLK2 Antibody (Pasritamig Biosimilar) with a linear range of 0.2-8 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Pasritamig captured on Protein A Chip can bind Human Kallikrein 2 Protein, His Tag (Cat. No. KA2-H52H3) with an affinity constant of 5.70 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity
Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is >150 pmol/min/μg (QC tested).
Protocol
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