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Product Details
Synonym
KLK2A2, hGK-1, hK2
Source
Biotinylated Human Kallikrein 2 Protein, His,Avitag (KA2-H82E3) is expressed from human 293 cells (HEK293). It contains AA Ile 25 - Pro 261 (Accession # P20151-1).
Predicted N-terminus: Ile 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 29.7 kDa. The protein migrates as 32-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Biotinylated Human Kallikrein 2 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-TR-FRET
In this TR-FRET assay, Streptavidin Protein Europium chelate (for TR-FRET) (Cat. No. FRT-TD09) is used as the Donor and Monoclonal Anti-Human IgG Antibody FA-Labeled (for TR-FRET) (Cat. No. FRT-TA02) is used as the Acceptor. Biotinylated Human Kallikrein 2 Protein, His,Avitag (Cat. No. KA2-H82E3), at 0.3 ng/well, can bind to the Pasritamig with a linear detection range of 8-0.016 μg/mL (Routinely tested).
In this TR-FRET assay, Streptavidin Protein Europium chelate (for TR-FRET) (Cat. No. FRT-TD09) is used as the Donor and Monoclonal Anti-Flag Tag Antibody FA-Labeled (for TR-FRET) (Cat. No. FRT-TA06) is used as the Acceptor. Human CD3E&CD3D Heterodimer Protein (Cat. No. CDD-H52Wa), at 10 ng/well and Biotinylated Human Kallikrein 2 Protein, His,Avitag (Cat. No. KA2-H82E3), at 5 ng/well, can bind to the Pasritamig with a linear detection range of 4-0.016 μg/mL (Routinely tested).
Bioactivity
Measured by its ability to cleave a fluorogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is >255 pmol/min/μg (QC tested).
Protocol
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