Détails du produit
Synonym
KLK3, Kallikrein-3, APS, KLK2A1, PSA, Hk3, Seminin, Semenogelase
Source
Cynomolgus Kallikrein 3 Protein, His Tag (KL3-C52H3) is expressed from human 293 cells (HEK293). It contains AA Pro 19 - Pro 261 (Accession # Q6DT45).
Predicted N-terminus: Pro 19
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 28.7 kDa. The protein migrates as 30-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Affichage des données
SDS-PAGE

Cynomolgus Kallikrein 3 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Cynomolgus Kallikrein 3 Protein, His Tag (Cat. No. KL3-C52H3) is more than 95% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.
Report
Bioactivity
Measured by its ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p-Nitroanilide (Suc-RPY-pNA). The specific activity is >15 pmol/min/μg (QC tested).
Protocol
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