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Cynomolgus Kallikrein 3 Protein, His Tag (active enzyme, MALS verified)

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Numéro de produit/spécification
Prix
Qté
KL3-C52H3-100ug
$368.00
KL3-C52H3-1mg (500ug X 2)
$2625.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Synonym

    KLK3, Kallikrein-3, APS, KLK2A1, PSA, Hk3, Seminin, Semenogelase

  • Source

    Cynomolgus Kallikrein 3 Protein, His Tag (KL3-C52H3) is expressed from human 293 cells (HEK293). It contains AA Pro 19 - Pro 261 (Accession # Q6DT45).

    Predicted N-terminus: Pro 19

    Request for sequence
  • Molecular Characterization

    Kallikrein 3 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 28.7 kDa. The protein migrates as 30-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • SDS-PAGE

    Kallikrein 3 SDS-PAGE

    Cynomolgus Kallikrein 3 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Kallikrein 3 SEC-MALS

    The purity of Cynomolgus Kallikrein 3 Protein, His Tag (Cat. No. KL3-C52H3) is more than 95% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.

    Report
  • Bioactivity

    Measured by its ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p-Nitroanilide (Suc-RPY-pNA). The specific activity is >15 pmol/min/μg (QC tested).

    Protocol

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Introduction de fond

Kallikrein-related peptidase 3, more commonly known as Kallikrein 3 (KLK3) or Prostate-Specific Antigen (PSA), is a serine protease belonging to the kallikrein family. Its primary function is to cleave proteins, and it is a key biomarker for prostate cancer. Secreted by the epithelial cells of the prostate gland, elevated levels of KLK3 in the blood are often indicative of prostate pathologies, including benign prostatic hyperplasia and prostate cancer. Beyond its role as a diagnostic marker, recent research explores its potential as a therapeutic target in cancer treatment. Its unique expression pattern and enzymatic function make it a subject of extensive research in oncology.

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