Human Kallikrein 5 Protein, Tag Free (active enzyme)

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Cat. No. / Size
Price
Qty
KA5-H5213-100ug
$350.00
KA5-H5213-1mg
$2500.00
ETA of in-stock products:2 business days

Product Details

  • Synonym

    KLK-L2, KLKL2, SCTE

  • Source

    Human Kallikrein 5 Protein, Tag Free (KA5-H5213) is expressed from human 293 cells (HEK293). It contains AA Ile 67 - Ser 293 (Accession # Q9Y337-1).

    Predicted N-terminus: Ile 67

    Request for sequence
  • Molecular Characterization

    Kallikrein 5 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries no "tag".

    The protein has a calculated MW of 25.2 kDa. The protein migrates as 33-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Kallikrein 5 SDS-PAGE

    Human Kallikrein 5 Protein, Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity

    Measured by its ability to cleave the fluorogenic peptide substrate BOC-Val-Pro-Arg-AMC. The specific activity is >350 pmol/min/μg (QC tested).

    Protocol

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Background

Kallikrein 5(KLK5), a serine protease encoded by the KLK5 gene, regulates skin desquamation by activating kallikrein proteases (e.g., KLK7) and degrading corneodesmosomes. In cancer, KLK5 exhibits dual roles: while overexpressed in colorectal and pancreatic cancers promotes metastasis via ECM degradation, its elevated expression in breast cancer suppresses tumor growth by inhibiting RhoA and modulating the mevalonate pathway. Dysregulated KLK5 activity drives inflammatory skin disorders through excessive cleavage of cathelicidins. Therapeutic strategies include covalent inhibitors for inflammatory diseases and KLK5-enhancing approaches in breast cancer. Serum KLK5 levels serve as biomarkers for fibrosis and cancer prognosis.

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