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Product Specifications
Assay TypeSandwich-ELISAAnalyteFLT3LFormat96T (8×12 strips)ReactivityHumanRegulatory StatusRUOSensitivity＜15.625 pg/mLStandard Curve Range15.625 pg/mL-500 pg/mLAssay Time3 hr 20 minSuitable Sample TypeFor the quantitative determination of human FLT3L in human serum and cell culture supernatant.Sample volume100 μLProduct Overview
Human FLT3L ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is used to detect and quantitatively determine GMP human FLT3L residues in cell culture supernatants, serum, and plasma. The kit contains GMP human FLT3L (ACROBiosystems, cat# GMP-FLLH28) to ensure accurate assay results and is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages. It can also be used as a universal detection tool for the quantitative determination of human FLT3L.
Reconstitution
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeRES007-C01Pre-coated Anti-FLT3L Antibody Microplate1 plate (8×12 strips)RES007-C02Human FLT3L Standard20 μgRES007-C03Biotin-Anti-FLT3L Antibody20 μgRES007-C04Streptavidin-HRP50 μLRES007-C0510×Washing Buffer50 mLRES007-C062×Dilution Buffer50 mLRES007-C07Substrate Solution12 mLRES007-C08Stop Solution7 mLACRO Quality Management System
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
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Drug Development Progress- English Name：
Fms-related tyrosine kinase 3 ligand
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