CHO/Human LIGHT Stable Cell Line

Cat. No. / Size
Price
Qty
SCCHO-ATP109-2Vials (1Vial X 2)
$5355.00
ETA of in-stock products:2 business days

Product Details

    1. Genetically modified cell lines best reflect MOA (Mechanism of Action)
    2. Higher activity and larger assay window for robust and reproducible cell-based bioassay
    3. Comprehensive application data to support assay development and validation
    4. Full tracible record, stringent quality control and validated cell passage stability
    5. Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
    6. Global commercial license assistance whenever regulatory filing is required
  • Description

    The CHO/Human LIGHT Stable Cell Line was engineered to express full length human LIGHT (Uniprot: O43557-1). Surface expression of human LIGHT was confirmed by flow cytometry.

  • Application

    • Useful for cell-based LIGHT binding assay

    • Useful as LIGHT-expressing target cells in reporter gene assay

    LIGHT Assay Principles

  • Growth Properties

    Adherent

  • Selection Marker

    Puromycin (2 μg/mL)

  • Complete Growth Medium

    F-12K + 10% FBS

  • Freeze Medium

    Serum-free cell cryopreservation medium

  • Quantity

    1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

  • Storage

    Frozen in liquid nitrogen.

  • Mycoplasma Testing

    Negative

  • Sterility Testing

    Negative

  • Instructions for Use

    See data sheet for detailed culturing and assay protocol.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

Customers Also Viewed

Performance Data

  • Receptor Assay

     LIGHT FACS

    Expression analysis of human LIGHT on CHO/Human LIGHT Stable Cell Line by FACS.
    CHO/Human LIGHT Stable Cell Line or negative control cell were stained with PE-labeled anti-Human LIGHT

    Protocol
  • Signaling Bioassay

     LIGHT SIGNALING

    Response to human HVEM (FOLD).
    This cell was incubated with Human HVEM (Luc) HEK293 Reporter Cell (Cat. No. CHEK-ATF105). The human LIGHT overexpressing on CHO cells can activate HVEM signaling with the max induction fold 227.59.

    Protocol
  • Passage Stability

     LIGHT PASSAGE

    Passage stability analysis of receptor expression by FACS.
    Flow cytometry surface staining of human LIGHT on CHO/Human LIGHT Stable Cell Line demonstrates consistent mean fluorescent intensity across passage 4-24

    Protocol
*Please contact us if you are interested in related cell pool service.

Customer Reviews
Writing Reviews

Important Statement

  • Limited Use&License Disclosure

    BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE FOLLOWING TERMS OF LIMITED USE OF THIS CELL LINE PRODUCT.

    1. If the researcher is not willing to accept the terms of limited use of this cell line product, and the product is unused, ACRO will accept return of the unused product.
    2. Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for profit or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research.
    3. This cell line is neither intended for any animal or human therapeutic purposes nor for any direct human in vivo use. You have no right to share, modify, transfer, distribute, sell, sublicense, or otherwise make the cell line available for use to other researchers, laboratories, research institutions, hospitals, universities, or service organizations.
    4. ACROBIOSYSTEMS MAKES NO WARRANTIES OR REPRESENTATIONS OF ANY KIND, EITHER EXPRESSED OR IMPLIED, WITH RESPECT TO THE SUITABILITY OF THE CELL LINE FOR ANY PARTICULAR USE.
    5. ACROBIOSYSTEMS ACCEPTS NO LIABILITY IN CONNECTION WITH THE HANDLING OR USE OF THE CELL LINE.
    6. Modifications of the cell line, transfer to a third party, or commercial use of the cell line may require a separate license and additional fees. Please contact order@acrobiosystems.com for further details.

Recent Advances

 
Drug Development Progress
  • English Name:

    Tumor necrosis factor superfamily member 14

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    2 Details

  • Highest Development Stage:

    Phase 2 Clinical

Drug Candidate Licensing
More
  • Basic Product Information
  • Product Details
  • Customers Also Viewed
  • Performance Data
  • Customer Reviews
  • Important Statement