Product Details
- Genetically modified cell lines best reflect MOA (Mechanism of Action)
- Higher activity and larger assay window for robust and reproducible cell-based bioassay
- Comprehensive application data to support assay development and validation
- Full tracible record, stringent quality control and validated cell passage stability
- Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
- Global commercial license assistance whenever regulatory filing is required
Description
The CHO/Human LIGHT Stable Cell Line was engineered to express full length human LIGHT (Uniprot: O43557-1). Surface expression of human LIGHT was confirmed by flow cytometry.
Application
• Useful for cell-based LIGHT binding assay
• Useful as LIGHT-expressing target cells in reporter gene assay

Growth Properties
Adherent
Selection Marker
Puromycin (2 μg/mL)
Complete Growth Medium
F-12K + 10% FBS
Freeze Medium
Serum-free cell cryopreservation medium
Quantity
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
Storage
Frozen in liquid nitrogen.
Mycoplasma Testing
Negative
Sterility Testing
Negative
Instructions for Use
See data sheet for detailed culturing and assay protocol.
ACRO Quality Management System
Customers Also Viewed
Performance Data
Receptor Assay

Expression analysis of human LIGHT on CHO/Human LIGHT Stable Cell Line by FACS.
CHO/Human LIGHT Stable Cell Line or negative control cell were stained with PE-labeled anti-Human LIGHTProtocol
Signaling Bioassay

Response to human HVEM (FOLD).
This cell was incubated with Human HVEM (Luc) HEK293 Reporter Cell (Cat. No. CHEK-ATF105). The human LIGHT overexpressing on CHO cells can activate HVEM signaling with the max induction fold 227.59.Protocol
Passage Stability

Passage stability analysis of receptor expression by FACS.
Flow cytometry surface staining of human LIGHT on CHO/Human LIGHT Stable Cell Line demonstrates consistent mean fluorescent intensity across passage 4-24Protocol
Customer Reviews Writing Reviews

Important Statement
Limited Use&License Disclosure
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE FOLLOWING TERMS OF LIMITED USE OF THIS CELL LINE PRODUCT.
- If the researcher is not willing to accept the terms of limited use of this cell line product, and the product is unused, ACRO will accept return of the unused product.
- Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for profit or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research.
- This cell line is neither intended for any animal or human therapeutic purposes nor for any direct human in vivo use. You have no right to share, modify, transfer, distribute, sell, sublicense, or otherwise make the cell line available for use to other researchers, laboratories, research institutions, hospitals, universities, or service organizations.
- ACROBIOSYSTEMS MAKES NO WARRANTIES OR REPRESENTATIONS OF ANY KIND, EITHER EXPRESSED OR IMPLIED, WITH RESPECT TO THE SUITABILITY OF THE CELL LINE FOR ANY PARTICULAR USE.
- ACROBIOSYSTEMS ACCEPTS NO LIABILITY IN CONNECTION WITH THE HANDLING OR USE OF THE CELL LINE.
- Modifications of the cell line, transfer to a third party, or commercial use of the cell line may require a separate license and additional fees. Please contact order@acrobiosystems.com for further details.
Recent Advances
- English Name:
Tumor necrosis factor superfamily member 14
- Category:
- Approved Drugs:
0 Details
- Drugs in Clinical Trials:
2 Details
- Highest Development Stage:
Phase 2 Clinical




















