IgG Antibody Isotype Controls
Antibody drugs have become an important field in the development of biomedicine. Isotype control antibodies in antibody drug development are of the same class and subtype as the primary antibody developed against the target, but do not specifically bind to a known or potential target. The purpose of using the isotype control is to confirm that the binding or effect of the primary antibody is specific, excluding non-specific interactions with Fc receptor proteins or with other proteins. Meanwhile, isotype control can also be used as an antibody that competes for binding to Fc receptor proteins, which performs the same function as functional blocking antibodies, which facilitates effective and reasonable evaluation of the efficacy of antibody drugs. Non-specific background is an important variable that needs to be controlled. Setting the corresponding isotype control antibody to detect non-specific binding can reduce the generation of false positive results and evaluate the possible influencing factors accurately in the drug development process.
We offer high-performance wild-type and mutant antibody isotype controls designed to accelerate your research. The wild-type Fc maintains its native conformation for reliable foundational studies and control experiments. And our mutant Fc is engineered with clinically relevant modifications that precisely match the engineering requirements of over 20 marketed therapeutic antibody. These rigorously validated isotype controls provide high specificity and reproducibility with minimal background interference, supporting reliable performance across FACS, ELISA, and SPR applications. Whether you're conducting basic research or developing next-generation biologics, our isotype controls provide the critical standardization needed to advance your antibody discovery, validation, and therapeutic development workflows with confidence.
Product Features
Dual Fc Design - Flexible for Your Needs
Wild-type Fc: Preserves native conformation, ideal for high-confidence controls
Mutant Fc: Engineered to match 20+ approved therapeutic antibodies
High Reproducibility
Rigorous QC ensures batch-to-batch consistency
No endogenous background for reliable results
Ready-to-Use Multi-Tag System
Direct compatibility with major platforms (FACS/ELISA/SPR)
CommanderVerification Data
High biological activity verified by ELISA
Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Human IgG1 (L234F,L235E,P331S) Kappa Isotype Control (mAb) (Cat. No. DNP-C11P1) with a linear range of 0.2-5 ng/mL (QC tested).
Immobilized Dinitrophenyl-BSA Conjugate (DNP-BSA) at 10 μg/mL (100 μL/well) can bind Human IgG2 (A330S, P331S) Kappa Isotype Control (mAb) (Cat. No. DNP-M884) with a linear range of 0.05-2 ng/mL (QC tested).
Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Monoclonal Anti-DNP antibody, Human IgG3 Isotype Control (Cat. No. DNP-M915) with a linear range of 0.1-4 ng/mL (QC tested).
Immobilized Dinitrophenyl-BSA Conjugate (DNP-BSA) at 10 μg/mL (100 μL/well) can bind Human IgG4 (S228P, L235E) Kappa Isotype Control (mAb) (Cat. No. DNP-M886) with a linear range of 0.02-2 ng/mL (QC tested).
FACS verified for use as isotype controls
2e5 of anti-CD19 CAR-293 cells were stained with 100ul of 1:50 dilution of 2e5 of anti-CD19 CAR-293 cells were stained with 100ul of 1:50 dilution of Mouse IgG1 kappa Isotype Control (Cat. No. DNP-M1A1) and positive control antibody respectively, washed and then followed by PE anti-mouse IgG1 and analyzed with FACS (QC tested).
2e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) Biotinylated Mouse IgG1 kappa Isotype Control (Cat. No. DNP-BM190) and positive control antibody (Cat. No. FM3-BY54) respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).
2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of FITC-Labeled Mouse IgG1 Isotype Control (Cat. No. DNP-FM1A1) and positive control (Cat. No. FM3-FY45P1) respectively, and FITC signal was used to evaluate the binding activity (QC tested).
5e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Monoclonal Mouse IgG1 Isotype Control (Cat. No. DNP-PM1) and positive control (Cat. No. FM3-HPY53) respectively, and PE signal was used to evaluate the binding activity (QC tested).
Other Isotype Controls
FAQ
How do I choose the appropriate isotype control for my antibody?
Why should an isotype control match the IgG subclass of the test antibody?
When should I use a wild-type or Fc-mutant isotype control?
How do I select an isotype control for an Fc-engineered therapeutic antibody?
Can these IgG isotype controls be used in flow cytometry?
Can these isotype controls be used in ELISA and SPR assays?
How do isotype controls help distinguish specific binding from non-specific background?
What should I consider if my isotype control shows a high background signal?
- Introduction
- Product Features
- Product List
- Verification Data
- Other Isotype Controls
- FAQ