Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09)

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218-MY2203-25ug
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218-MY2203-100ug
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ETA of in-stock products:2 business days

Product Details

  • Source

    Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09) is a monoclonal antibody recombinantly expressed from HEK293 cells, which provides higher batch consistency and long term security of supply.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    P3C09

  • Isotype

    Rabbit IgG, Kappa

  • Host Species

    Rabbit

  • Reactivity

    Human

  • Immunogen

    Purified 218 linker protein

  • Specificity

    This product is a specific antibody specifically reacts with CAR or scFv containing the 218 linker sequence.

  • Application

    Flow Cytometry (Evaluation of CAR and SCFV containing 218linker on Human cells).

  • Recommended Dilution

    1:50

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 12 months after reconstitution.
    3. 2-8 °C for 12 months after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     218 linker ELISA

    Immobilized FMC63 scFv, His Tag at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09) (Cat. No. 218-MY2203) with a linear range of 0.06-1 ng/mL (QC tested).

    Protocol

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Background

The whitlow/218 linker, with the sequence GSTSGSGKPGSGEGSTKG, is a commonly used synthetic peptide linker. Whitlow/218 linker often leveraged to connect the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The scFv containing the 218 linker showed reduced aggregation and was found to be more stable to proteolysis in vitro.

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