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Background
Recombinant Monoclonal Anti-CD68 Antibody, Rabbit (2D8) is a CD68 primary antibody for IHC application. Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Product Specifications
Host SpeciesRabbitIsotypeRabbit IgG, Rabbit KappaClone2D8ApplicationIHC-PDilution Ratio1: 500 - 1: 2000FormLiquidClonalityMonoclonalReactivityHumanSynonymsDKFZp686M18236, GP110, LAMP4, macrosialin, SCARD1Concentration0.9~1.1 mg/mlSpecificityReact with CD68 protein on the membrane of target cell/tissuePurification TechProtein APreservationPBS, BSA, GlycerolExperiment Protocol
Storage
Shipped at 4°C, Blue Ice. Store at +4°C for short term (1-2 weeks). Store at -20°C for long term.
Avoid freeze / thaw cycle. Upon delivery aliquot. The shelf life is 30 days from the date of opening.ACRO Quality Management System
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Sample Staining Data
Immunohistochemical analysis of paraffin embedded human colorectal cancer tissue labelled with HGS-S262 at 1/1000 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with LEICA BOND IHC staining protocol.
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