COA
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Monoclonal Anti-G4S linker Antibody, Mouse IgG1 (AM635) is a mouse monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply. APC-Labeled Monoclonal Anti-G4S linker Antibody, Mouse IgG1 (AM635) is produced via site-specific conjugation of APC to Monoclonal Anti-FMC63 Antibody, Mouse IgG1 under optimal conditions with a proprietary technology.
Flow Cytometry (Evaluation of cell surface expressed CARs of varying specificity containing a G4S linker within the scFv of the extracellular domain). Please note that this product is NOT compatible to streptavidin detection system.
AM635
Mouse
Mouse IgG1/kappa
Specifically recognizes the scFv-based CARs containing a G4S linker.
APC
Excitation Wavelength: 640 nm
Emission Wavelength: 661 nm
1:25
Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
The G4S linker is emerged as the most common linking peptides for scFvs. It is composed of the repeated sequence (Gly-Gly-Gly-Gly-Ser) and commonly found as either a 15-mer (G4S)3 or 20-mer(G4S)4 within scFv fragments. The G4S linker has become an integral part of the vast majority of CARs as it links the VH and VL recognition domains of scFvs. The G4S linker is commonly used in CARs targeting solid tumor antigens, such as HER2 and CEA, while the whitlow/218 linker peptide is predominantly used in CAR constructs targeting hematological malignancies such as CD19 CAR products.
