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Détails du produit
Source
Monoclonal Anti-HA (Influenza A/Darwin/9/2021 (H3N2)) Antibody, Mouse IgG1 (1D6) is a mouse monoclonal antibody derived from hybridoma cells fused with SP2/0 myeloma cells and murine B lymphocytes.
Antibody Type
Hybridoma Monoclonal
Clone
1D6
Isotype
Mouse IgG1, Kappa
Host Species
Mouse
Reactivity
Virus
Immunogen
Recombinant Influenza A [A/Darwin/9/2021 (H3N2)] HA Protein is expressed from human 293 cells
Specificity
Specifically recognizes HA (Influenza A/Darwin/9/2021 (H3N2)).
Application
ApplicationRecommended UsageELISA0.2-2 μg/mLCross Verification
This product No cross-reactivity in ELISA with
Influenza A [Victoria/4897/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H8).
Influenza A [Wisconsin/67/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H7).
Influenza A [A/Hong Kong/483/97 (H5N1)] HA, His Tag (Cat. No. HA1-V5229).
Influenza A (Vietnam/1194/2004(H5N1)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H9).
Influenza A (Guangdong/18SF020(H5N6)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA6-V52H3).
Influenza A (turkey/Germany-MV/R2472/2014(H5N8)) HA Protein, His Tag (Cat. No. HA8-V52H3).
Influenza A (A/Shanghai/02/2013(H7N9)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA9-V52H3).
Influenza B [Phuket/3073/2013 (B/Yamagata lineage)] HA Protein, His Tag (Cat. No. HAE-V52H4).
This product No cross-reactivity in ELISA with
Influenza A Virus (A/District of Columbia/27/2023) HA (H3N2) Protein, His Tag (Cat. No. H32-V52H5).
Influenza A Virus (A/Croatia/10136RV/2023) HA (H3N2) Protein, His Tag (Cat. No. H32-V52H4).Purification
Protein A purified / Protein G purified.
Aggregation
Less than 5%, as determined by SEC-MALS.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.0), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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Affichage des données
Bioactivity-ELISA
Immobilized Influenza A [A/Darwin/9/2021 (H3N2)] Hemagglutinin (HA) Protein, His Tag (MALS verified) (Cat. No. HA2-V52H6) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-HA (Influenza A/Darwin/9/2021 (H3N2)) Antibody, Mouse IgG1 (1D6)(Cat. No. HA2-Y196) with a linear range of 0.195-6.25 ng/mL (QC tested).
Protocol
Immobilized Influenza A [A/Darwin/9/2021 (H3N2)] Hemagglutinin (HA) Protein, His Tag (MALS verified) (Cat. No. HA2-V52H6) can bind Monoclonal Anti-HA (Influenza A/Darwin/9/2021 (H3N2)) Antibody, Mouse IgG1 (1D6)(Cat. No. HA2-Y196). The antibody does not bind Influenza A [A/Bangkok/1/1979 (H3N2)] Hemagglutinin (HA) Protein, His Tag (MALS verified)(Cat. No. HA2-V52H3), Influenza A [A/Wisconsin/588/2019 (H1N1)] Hemagglutinin (HA) Protein, His Tag (MALS verified)(Cat. No. HA1-V52H3), Influenza A [A/Darwin/6/2021 (H3N2)] Hemagglutinin (HA) Protein, His Tag (MALS verified)(Cat. No. HA2-V52H5), Influenza A [Sydney/5/2021 (H1N1)] Hemagglutinin (HA) Protein, His Tag (MALS verified)(Cat. No. HA1-V52H4), Influenza B [Austria/1359417/2021 (B/Victoria lineage)] Hemagglutinin (HA) Protein, His Tag(Cat. No. HAE-V52H3), Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] Hemagglutinin 1 (HA1) Protein, His Tag (MALS verified)(Cat. No. HA1-V52H5), Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] Hemagglutinin (HA) Protein, His Tag (MALS verified)(Cat. No. HA2-V52H7) and Influenza A [A/Victoria/2570/2019] Hemagglutinin (HA) Protein, His Tag (MALS verified)(Cat. No. HA1-V52H6). (Routinely tested).
Protocol
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