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Détails du produit
Source
Monoclonal Anti-His Antibody, Human IgG1 (55F8) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain, which provides higher batch consistency and long term security of supply.
Antibody Type
Recombinant Monoclonal
Clone
55F8
Isotype
Human IgG1, Kappa
Host Species
Mouse
Reactivity
His tag (N‑terminal, C‑terminal, internal His‑tagged fusion proteins)
Immunogen
A protein peptide with his tag
Conjugate
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
Specificity
This product is a specific antibody specifically reacts with His tag protein, and can recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
Application
Flow Cytometry (Detection the His-tagged protein).
Recommended Dilution
1:50
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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Affichage des données
Bioactivity-Flow Cytometry
Flow cytometric analysis of Anti-CD19 CAR-293 cells staining with 100 μL of 10 μg/mL Human CD19 (20-291) Protein, His Tag (Cat. No. CD9-H52H2) and negative control protein respectively, washed and then followed with Alexa Fluor 488-Labeled Monoclonal Anti-His Antibody, Human IgG1 (55F8) (Cat. No. HIS-AFM549) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL) and analyzed with FACS. Alexa Fluor 488 signal was used to evaluate the binding activity (QC tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
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