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Monoclonal Anti-His Antibody, Human IgG1 (AY63)

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Numéro de produit/spécification
Prix
Qté
HIS-M535-100ug
$245.00
HIS-M535-1mg
$1625.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Source

    Monoclonal Anti-His Antibody, Human IgG1 (AY63) is a recombinant chimeric monoclonal antibody expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    AY63

  • Isotype

    Human IgG1, Kappa

  • Host Species

    Mouse

  • Reactivity

    His tag (N-terminal, C-terminal and internal His-tagged fusion proteins)

  • Immunogen

    A protein peptide with his tag

  • Specificity

    This product is a specific antibody specifically reacts with His tag protein, and can recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.

  • Application

    Application
    Recommended Usage
    Western Blot
    0.2-1 ug/mL
    ELISA
    0.2-2 μg/mL
  • Purification

    Protein A purified / Protein G purified.

  • Aggregation

    Less than 10%, as determined by SEC-MALS.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Affichage des données

  • Bioactivity-ELISA

     His ELISA

    Immobilized Canine TNF-alpha, His Tag (Cat. No. TNA-C52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-His Antibody, Human IgG1 (AY63) (Cat. No. HIS-M535) with a linear range of 0.1-1 ng/mL (QC tested).

    Protocol
  • Bioactivity-SPR

     His SPR

    Monoclonal Anti-His Antibody, Human IgG1 (AY63) (Cat. No. HIS-M535) captured on Protein G Chip can bind Rabbit Serum Albumin, His Tag (Cat. No. RSA-R52H6) with an affinity constant of 29.6 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol
  • Western Blot

     His WESTERN BLOT
  • Detection of Monoclonal Anti-His Tag Antibody, Human IgG1 (AY63), mAb by Western Blot. Monoclonal Anti-His Tag Antibody, Human IgG1 (AY63), mAb at 1/20000 dilution + Human LAG-3 / CD223 Protein , His Tag at 75-250ng.
    Secondary Antibody: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) at 1/2000 dilution.
    Predicted band size: 60 kDa 12% Bis-Tris Protein Gel.

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Introduction de fond

His tag is a very small molecular weight tag, usually composed of 6-10 histidine (His). It is one of the commonly used tags for protein purification and detection. Due to its small molecular weight, fusion into the target protein has almost no effect on the structure and characteristics of the protein. Anti-his tag antibody can accurately detect, locate and purify His tag fusion protein, so as to provide convenience for the vast number of researchers.

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