Détails du produit
Source
Monoclonal Anti-His Antibody, Human IgG1 (AY63) is a recombinant chimeric monoclonal antibody expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.
Antibody Type
Recombinant Monoclonal
Clone
AY63
Isotype
Human IgG1, Kappa
Host Species
Mouse
Reactivity
His tag (N-terminal, C-terminal and internal His-tagged fusion proteins)
Immunogen
A protein peptide with his tag
Specificity
This product is a specific antibody specifically reacts with His tag protein, and can recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
Application
ApplicationRecommended UsageWestern Blot0.2-1 ug/mLELISA0.2-2 μg/mLPurification
Protein A purified / Protein G purified.
Aggregation
Less than 10%, as determined by SEC-MALS.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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Affichage des données
Bioactivity-ELISA

Immobilized Canine TNF-alpha, His Tag (Cat. No. TNA-C52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-His Antibody, Human IgG1 (AY63) (Cat. No. HIS-M535) with a linear range of 0.1-1 ng/mL (QC tested).
Protocol
Bioactivity-SPR

Monoclonal Anti-His Antibody, Human IgG1 (AY63) (Cat. No. HIS-M535) captured on Protein G Chip can bind Rabbit Serum Albumin, His Tag (Cat. No. RSA-R52H6) with an affinity constant of 29.6 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Western Blot

Detection of Monoclonal Anti-His Tag Antibody, Human IgG1 (AY63), mAb by Western Blot. Monoclonal Anti-His Tag Antibody, Human IgG1 (AY63), mAb at 1/20000 dilution + Human LAG-3 / CD223 Protein , His Tag at 75-250ng.
Secondary Antibody: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) at 1/2000 dilution.
Predicted band size: 60 kDa 12% Bis-Tris Protein Gel.
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