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Human IgG1 (N297A) Kappa Isotype Control, premium grade (mAb, carrier free)

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Numéro de produit/spécification
Prix
Availability
Qté
DNP-MB273-100ug
$190.00
In Stock - Delivery in 2-3 business days
DNP-MB273-1mg (250ug X 4)
$830.00
In Stock - Delivery in 2-3 business days
DNP-MB273-25mg
$1855.00
DNP-MB273-100mg
$6180.00
Total des articles Montant du produit$ 0

Détails du produit

  • Source

    Human IgG1 (N297A) Kappa Isotype Control, premium grade (mAb) is a chimeric monoclonal antibody recombinantly expressed in HEK293 cells, which combines the variable regions of a mouse monoclonal antibody with human IgG1 (N297A) constant domains.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    C1484

  • Isotype

    Human IgG1 (N297A), Kappa

  • Host Species

    Mouse

  • Specificity

    This product is a specific antibody specifically reacts with DNP.

  • Application

    Application
    Recommended Use / Performance
    ELISA
    0.2-2 μg/mL
  • Purification

    Protein A purified.

  • Aggregation

    Less than 5%, as determined by SEC-MALS.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

    25 mg or larger size will be supplied as liquid and shipped by dry ice. Please inquire the dry ice shipping cost.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • Bioactivity-ELISA

     IgG control ELISA

    Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Human IgG1 (N297A) Kappa Isotype Control, premium grade (mAb) (Cat. No. DNP-MB273) with a linear range of 0.1-1 ng/mL (QC tested).

    Protocol

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FAQ

  • product

    Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?

    In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.


    Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.


    To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.


    To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.


    Supporting Document


Introduction de fond

A hapten is a small molecule that can elicit an immune response only when conjugated with a large carrier such as a protein. Typical haptens include drugs, urushiol, quinone, steroids, etc. Peptides and non-protein antigens usually need conjugating to a carrier protein (such as BSA (bovine serum albumin) or KLH (keyhole limpet hemocyanin) to become good immunogens). Additionally, haptens should be administered with an adjuvant to ensure a high quality immune response. It is important that the hapten design (preserving greatly the chemical structure and spatial conformation of target compound), selection of the appropriate carrier protein and the conjugation method are key conditions for the desired specificity anti-hapten antibodies. We design anti-hapten antibodies based on the HaptenDB information.

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