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Détails du produit
Source
Monoclonal Rabbit IgG Antibody Isotype Control is a chimeric monoclonal antibody recombinantly expressed in HEK293 cells, which combines the variable region of a mouse monoclonal antibody with rabbit IgG constant domain.
Antibody Type
Recombinant Monoclonal
Clone
AM776
Isotype
Rabbit IgG, Kappa
Host Species
Mouse
Immunogen
DNP (Dinitrophenyl)
Conjugate
AF555
Excitation Wavelength: 561 nm
Emission Wavelength: 572 nm
Specificity
Specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins, which is normally not present in Humans or animals. Therefore, IGG-AEFM776 does not react with any antigen of Human or animal cells.
Application
Flow Cytometry.
Recommended Dilution
1:50
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months after reconstitution.
- 2-8 °C for 12 months after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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Affichage des données
Bioactivity-Flow Cytometry
Flow cytometric analysis of Anti-MSLN CAR-293 staining with Alexa Fluor 555-Labeled Rabbit IgG Antibody Isotype Control (Cat. No. IGG-AEFM776) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with positive control antibody. Alexa Fluor 555 signal was used to evaluate the binding activity (QC tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
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