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Monoclonal Anti-Human IgG Fc Antibody, Mouse IgG1 (6F11C8) is a recombinant mouse monoclonal antibody expressed in CHO cells.
Recombinant Monoclonal
6F11C8
Mouse IgG1, Kappa
Mouse
Human IgG Fc, including IgG1–IgG4 isotypes and Fc-engineered variants (e.g. N297A, LALA, YTE, LS, S239D/I332E)
Recombinant Human Fc derived from CHO cells
Specifically binds to the Fc region of human IgG without recognizing Fab fragments or mouse IgG isotypes.
Application | Recommended Use |
ELISA | 0.2-2 μg/mL |
* Recommended dilutions are for reference only. Optimal working concentrations may vary depending on your consumables, reagents, and assay conditions. We recommend optimizing for your specific system. No warranty is made regarding performance at any specific dilution.
Positive: Cross-reactive by ELISA with human IgG subclasses (IgG1, IgG2, IgG3, IgG4) and a panel of Fc-engineered variants including N297A, LALA, YTE, LS, S239D/I332E. Weak cross-reactivity by ELISA with cynomolgus IgG.
Negative: No detectable cross-reactivity by ELISA with mouse IgG subclasses (IgG1, IgG2a, IgG2b).
Protein A purified.
Less than 10%, as determined by SEC-MALS.
Please refer to the Certificate of Analysis (CoA).
Lyophilized
Lyophilized from a 0.22 μm-filtered solution in PBS, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Lyophilized product is shipped at ambient temperature.
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
Product Specific Notices: For research use only.