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Application
The Monoclonal Anti-Human IgG Antibody FA-Labeled is a universal tool for TR-FRET assays that can bind human IgG or hFc-tagged proteins. This product uses specific anti-human IgG antibody labeled with fluorescent dyes and it can be used in combination with other Donors covalently conjugated with Eu³⁺ chelate. When the Donor and Acceptor come into close proximity (within a distance of less than 10 nm), a FRET reaction occurs: the 620 nm signal emitted by the Donor upon excitation by a specific light source is received by the Acceptor, which then emits a 665 nm signal.Product Specifications
SourceThis Monoclonal Anti-Human IgG Antibody is a mouse monoclonal antibody recombinantly expressed from Hybridoma.SpecificityThis antibody recognizes all four human IgG isotypes (IgG1, IgG2, IgG3, and IgG4) and multiple engineered variants, including: Human IgG1 (N297A), Human IgG1 (L234A, L235A), Human IgG1 (M252Y, S254T, T256E), Human IgG1 (L234F, L235E, P331S), Human IgG1 (S239D, I332E), Human IgG1 (S298A, E333A, K334A), Human IgG1 (T307A, E380A, N434A), Human IgG2 (A330S, P331S), Human IgG2 (P331S), Human IgG4 (S108P), and Human IgG4 (S228P, L235E).Cross-ReactivityWeak cross-reactivity with cynomolgus IgG, whereas no reactivity to human IgM, human IgA, mouse IgG isotypes (IgG1, IgG2a, IgG2b) or rabbit IgG.FormulationLyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.Physical AppearancePowderStorage (unopened)-20°C to -70°CStorage (opened)-70°CUnit Size1000 tests & 5000 tests & 20000 testsTechnologyTR-FRETIntended UseFor binding the human IgG antibody or human Fc tagged protein as acceptor in TR-FRET AssayReconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
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Performance Data
Bioactivity-TR-FRET
Please refer to DS document for the assay protocol.
In this TR-FRET assay, Streptavidin Protein Europium chelate (Cat. No. FRT-TD09) is used as the Donor and Monoclonal Anti-Human IgG Antibody FA-Labeled (Cat. No. FRT-TA02) is used as the Acceptor. Biotinylated Human PD-1 Protein, Avitag,His Tag (Cat. No. PD1-H82E4), at 1 ng/well, can bind to the Monoclonal Anti-Human PD-1 Antibody, Human IgG4 with a linear detection range of 5-0.005 μg/mL (QC tested).
In this TR-FRET assay, Streptavidin Protein Europium chelate (for TR-FRET) (Cat. No. FRT-TD09) is used as the Donor and Monoclonal Anti-Human IgG Antibody FA-Labeled (for TR-FRET) (Cat. No. FRT-TA02) is used as the Acceptor. Biotinylated Human Nectin-4, His,Avitag (Cat. No. NE4-H82E7), at 0.3125 ng/well, can bind to the Enfortumab with a linear detection range of 8-0.001 μg/mL (Routinely tested).
In this TR-FRET assay, Streptavidin Protein Europium chelate (for TR-FRET) (Cat. No. FRT-TD09) is used as the Donor and Monoclonal Anti-Human IgG Antibody FA-Labeled (for TR-FRET) (Cat. No. FRT-TA02) is used as the Acceptor. Biotinylated Human TROP-2, His,Avitag (Cat. No. TR2-H82E5), at 0.3125 ng/well, can bind to the Sacituzumab with a linear detection range of 4-0.001 μg/mL (Routinely tested).
In this TR-FRET assay, Streptavidin Protein Europium chelate (for TR-FRET) (Cat. No. FRT-TD09) is used as the Donor and Monoclonal Anti-Human IgG Antibody FA-Labeled (for TR-FRET) (Cat. No. FRT-TA02) is used as the Acceptor. Biotinylated Human CD3 epsilon, His,Avitag (Cat. No. CDE-H82E1), at 0.3125 ng/well, can bind to the Teclistamab with a linear detection range of 8-0.001 μg/mL (Routinely tested).
In this TR-FRET assay, Streptavidin Protein Europium chelate (for TR-FRET) (Cat. No. FRT-TD09) is used as the Donor and Monoclonal Anti-Human IgG Antibody FA-Labeled (for TR-FRET) (Cat. No. FRT-TA02) is used as the Acceptor. Biotinylated Human DLL3 (27-479) Protein, His,Avitag (Cat. No. DL3-H82Ea), at 0.5 ng/well, can bind to the Tarlatamab with a linear detection range of 4-0.002 μg/mL (Routinely tested).
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