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Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG3 (AM359b) (MALS verified)

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Numéro de produit/spécification
Prix
Qté
SPD-M401a-100ug
$450.00
SPD-M401a-1mg (500ug X 2)
$3255.00
ETA of in-stock products:2 business days
Total des articles Montant du produit$ 0

Détails du produit

  • Source

    The antibody is isolated from the serum of COVID-19 vaccine recipient and is recombinantly produced from human 293 cells (HEK293). This antibody can broadly neutralize SARS-CoV-2 Variants of Concerns (VOCs) including Alpha, Beta, Gamma, Delta and Omicron.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    AM359b

  • Isotype

    Human IgG3, Kappa

  • Specificity

    This product is a specific antibody against SARS-CoV-2 Spike protein RBD domain. Cross-reactivity with Spike protein RBD domain of other coronaviruses, including SARS-CoV, MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1 has not been tested.

  • Application

    Application
    Recommended Usage
    ELISA
    0.2-2 μg/mL
  • Purification

    Protein A purified / Protein G purified.

  • Aggregation

    Less than 10%, as determined by SEC-MALS.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Affichage des données

  • Bioactivity-ELISA

     Spike RBD ELISA

    Immobilized SARS-CoV-2 Spike Trimer, His Tag (B.1.1.529/Omicron) (Cat. No. SPN-C52Hz) at 2μg/mL (100μL/well) can bind Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG3 (AM359b) (Cat. No. SPD-M401a) with a linear range of 0.2-6 ng/mL (QC tested).

    Protocol
  •  Spike RBD ELISA

    Immobilized SARS-CoV-2 Spike protein WT (Cat. No. SPN-C52H9), Alpha (Cat. No. SPN-C52H6), Beta (Cat. No. SPN-C52Hk), Gamma (Cat. No. SPN-C52Hg), Delta (Cat. No. SPN-C52He) and Omicron (Cat. SPN-C52Hz) can bind Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG3 (AM359b) (Cat. No. SPD-M401a) with a linear range of 0.20-6.25 ng/mL (Routinely tested).

    Protocol
  •  Spike RBD ELISA

    Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG3 (AM359b) (Cat.No. SPD-M401a) neutralizes SARS-CoV-2 Spike Trimer by inhibiting Spike: ACE2 interaction. The Wild type (WT) Spike Trimer or Alpha, Delta, B.1.1.529/Omicron, BA.2/Omicron, BA.3/Omicron mutant-coated plate is incubated with the Biotinylated Human ACE2 / ACEH Protein and treated with the neutralizing antibody at increasing concentration . Percent inhibition is calculated based on the OD value.

    Protocol

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Introduction de fond

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

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  • Informations de base sur le produit
  • Détails du produit
  • Les clients ont également consulté
  • Affichage des données
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