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Détails du produit
- ItemsDetailsDetection MethodChemiluminescenceProduct TypeMagnetic Beads (Monoclonal anti-FITC antibody)Quantity Size10mg / 100mgPhysical Appearancelyophilized powder mixtureParticle size2.8µmBeads SurfaceHydrophilicBinding Capacity1-2μg FITC Antibody / mg beadsEmission WavelengthMeasured relative light units (RLU) at 430 nm
Product Description
The Anti-FITC Antibody coupled Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly affinity Monoclonal anti-FITC antibody. The beads can be used to capture the FITC Antibody in Chemiluminescence procedures. The Anti-FITC Antibody coupled Magnetic Beads is easy to capture the FITC labeled proteins, and the bounded proteins have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.
Application
The Anti- FITC Antibody coupled Magnetic Beads is used to capture the FITC Antibody, it can combination with Acridinium markers in chemiluminescence technology, The Acridinium ester markers such as Anti-Human IgG Antibody-Acridinium ester can capture the antibodies , this allows detection of antigen and antibody binding or antibody screening.
Reconstitution
See Certificate of Analysis (CoA) for detailed instruction.
Storage
Upon receipt, please store the Beads at -20℃. The shelf life is 1 year at -20 ℃.
Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
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Bioactivity-MPCLIA
Immobilized 0.05 μg /Test of FITC-Labeled Human BCMA, His Tag(Cat. No. BCA-HF2H1) to the Anti-FITC Antibody coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A009) (10 μg beads/Test), incubated with 100 μL /Test of Monoclonal Anti-Human BCMA Antibody, Human IgG1 (Cat. No. BCA-M26) at increasing concentration coupled to the Anti-Human IgG Antibody-Acridinium ester(Cat. No. AHG-Y69, 0.04 μg /Test). Detection was performed with sensitivity of 0. 98 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
Protocol
The MPCLIA assay shows that Anti-FITC Antibody coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A009) is stable at 37°C for 10 days.
The MPCLIA assay shows that Anti-FITC Antibody coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A009) is stable after freezing and thawing 3 times.
The MPCLIA assay shows that Anti-FITC Antibody coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A009) is stable at 37°C for 10 days.
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