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Détails du produit
- ItemsDetailsDetection MethodChemiluminescenceProduct TypeMagnetic Beads (Monoclonal anti-human IgM antibody)Quantity Size10mg / 100mgPhysical Appearancelyophilized powder mixtureParticle size2.8 µmBeads SurfaceHydrophilicBinding Capacity3-8 μg Human IgM / mg beadsEmission WavelengthMeasured relative light units (RLU) at 430 nm
Product Description
The Anti-Human IgM-coupled Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly affinity Monoclonal anti-human IgM antibody. The beads can be used to capture the Human IgM in Chemiluminescence procedures. It has no-corss-reactivity with Human IgG, IgA or IgE. The Anti-Human IgM-coupled Magnetic Beads is easy to capture the human IgM, and the bounded antibody have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.
Application
The Anti-Human IgM-coupled Magnetic Beads is used to capture the human IgM, it can combination with Acridinium ester markers in chemiluminescence technology, The Acridinium ester markers such as Streptavidin-Acridinium ester can capture the biotinylated proteins or molecules, this allows detection of antigen and antibody binding or antibody screening.
Reconstitution
See Certificate of Analysis (CoA) for detailed instruction.
Storage
Upon receipt, please store the Beads at -20℃. The shelf life is 1 year at -20 ℃.
Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
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Affichage des données
Bioactivity-MPCLIA
Immobilized 0.04 μg /Test of Biotinylated SARS-CoV-2 Spike RBD, His,Avitag (Cat. No. SPD-C82E9) to the Streptavidin Protein-Acridine ester (Cat. No. STN-NA114, 0.04 μg /Test), incubated with 100 μL /Test of Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgM (AM122) (Cat. No. SPD-M162) at increasing concentration coupled to Anti-Human IgM-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A010, 10 μg /Test). Detection was performed with sensitivity of 0.244 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
Protocol
The MPCLIA assay shows that Anti-Human IgM-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A010) is stable at 37°C for 10 days.
The MPCLIA assay shows that Anti-Human IgM-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A010) is stable after freezing and thawing 3 times.
The MPCLIA assay shows that Anti-Human IgM-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A010) is stable at 37°C for 10 days.
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