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Items | Size 2mg | Size 10mg (5mg × 2) |
Particle size | 2 μm | 2 μm |
Physical appearance | Powder mixture | Powder mixture |
Source | Gly 27 - Thr 461 (Accession # Q5ZPR3-1) | Gly 27 - Thr 461 (Accession # Q5ZPR3-1) |
Amount of Coupled Protein | ＞481 pmol (15 μg) B7-H3 (4Ig)/mg beads | ＞481 pmol (15 μg) B7-H3 (4Ig)/mg beads |
Formulation | PBS, pH7.4, with 10% Trehalose | PBS, pH7.4, with 10% Trehalose |
Reconstitution | 2 mL sterile deionized water (1 mg beads/mL) | 5 mL sterile deionized water (1 mg beads/mL) |
The Human B7-H3-coupled Magnetic Beads is produced by coupling biotinylated human B7-H3 protein to streptavidin-conjugated magnetic beads. The pre-coupled beads are ready to use for capturing anti-B7-H3 antibody from your sample with high specificity.
The beads is intended for immunocapture, biopanning. This is a non-sterile product.
* The Isotype control (Cat. No. SMB-B01) is sold separately.
See Certificate of Analysis (CoA) for detailed instruction.
This product is stable in storage under the following conditions: -20℃ for 24 months in lyophilized state.
Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
Antibody Purification:
1. Reconstitute the beads with ultrapure water to 1 mg/mL, and wash the beads for three times.
2. Dilute the biotinalyted protein or antibodies with Assay Buffer and add to the beads. Incubate for 60 minutes at room temperature in a rotary mixer.
3. Wash the beads for four times, and add Assay Buffer to the beads. The beads are ready to use for the next experiment.
The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.